RRC ID 29780
著者 Timmis K, Cabello F, Cohen SN.
タイトル Cloning, isolation, and characterization of replication regions of complex plasmid genomes.
ジャーナル Proc Natl Acad Sci U S A
Abstract EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras and the parent replicons has localized the cloned R6-5 replication region to a DNA segment between kilobase pair coordinates 1.0 and 88.0 on the R6-5 map. Physical proximity between the plasmid replication functions and the locus governing plasmid incompatibility has been shown for both parent replicons. The cloning method reported appears to be generally applicable for the identification and isolation of replication regions of a variety of complex genomes.
巻・号 72(6)
ページ 2242-6
公開日 1975-6-1
DOI 10.1073/pnas.72.6.2242
PMID 1094460
PMC PMC432733
MeSH Centrifugation, Density Gradient DNA Replication* DNA Restriction Enzymes DNA, Bacterial / isolation & purification* Electrophoresis, Agar Gel Escherichia coli / analysis* Escherichia coli / ultrastructure Extrachromosomal Inheritance* Genetics, Microbial Hybridization, Genetic Microscopy, Electron Molecular Weight Phenotype Plasmids* Selection, Genetic Staphylococcus Transformation, Genetic
IF 9.412
リソース情報
原核生物(大腸菌) ME6171 ME6172 ME6163