RRC ID 29781
Author Davies JK, Reeves P.
Title Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A.
Journal J Bacteriol
Abstract By using each of the available colicins, we have isolated a large number of colicin-resistant mutants. They included both receptor and tolerant mutants and each was screened for cross-resistance to all other colicins. On the basis of the cross-resistance of these mutants it was possible to place known colicins into two mutually exclusive groups, group A and group B. Mutants selected as resistant to colicins of group A may or may not be cross-resistant to other colicins of group A, BUT Are never resistant to colicins of group B. The reverse also applies. The mutants isolated as resistant to colicins of group A (A, E1, E2, E3, K, L, N, S4, and X) have been divided into 21 phenotypic classes on the basis of their colicin resistance patterns. These include most of the tolerant and receptor mutants previously isolated, some of which were previously shown to also have an increased sensitivity to certain antibiotics and detergents. Type strains from each of the phenotypic classes were therefore tested for sensitivity to a range of antibiotics, detergents, and surfactants that included all those previously used. With these new data, it has been possible to speculate informatively on the mode of action of the different colicins. We have confirmed the position of previously isolated mutations on the Escherichia coli K-12 genetic map, and located approximately the loci conferring colicin resistance in some of the newly isolated mutants.
Volume 123(1)
Pages 102-17
Published 1975-7-1
DOI 10.1128/jb.123.1.102-117.1975
PMID 1095546
PMC PMC235696
MeSH Anti-Bacterial Agents / pharmacology Chromosome Mapping Colicins / classification Colicins / pharmacology* Coliphages / metabolism Conjugation, Genetic Detergents / pharmacology Drug Resistance, Microbial* Escherichia coli / classification Escherichia coli / drug effects* Mutation Phenotype Surface-Active Agents / pharmacology Transduction, Genetic
IF 3.006
Prokaryotes E. coli ME6122 ME6123 ME6124 ME6125 ME6126 ME6127