論文 - 詳細
| RRC ID | 29795 |
|---|---|
| 著者 | Nagai H, Yuzawa H, Yura T. |
| タイトル | Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
When Escherichia coli cells are transferred from 30 degrees C to 42 degrees C, transcription from specific promoters recognized by RNA polymerase containing sigma 32 (the rpoH gene product) is transiently activated, resulting in induction of heat shock proteins. Transcription from heat shock promoters is activated by an increased cellular concentration of sigma 32 due to enhanced synthesis and stabilization. We have constructed and examined the expression of mutant derivatives (deletions and base substitutions) of rpoH-lacZ gene fusion. Synthesis of a sigma 32-beta-galactosidase fusion protein was found to be regulated at the translational level involving two distinct 5'-proximal rpoH coding regions. A small region immediately downstream of the initiation codon is required for potentially high-level expression, whereas a much larger internal region is required for thermal regulation--namely, repression at low temperature or nonstress conditions. The two mRNA regions act as positive and negative cis elements, respectively, in controlling rpoH translation. We propose that an interplay between these RNA regions involving secondary structure formation is important in regulating translation initiation and that transient disruption of secondary structure represents a primary step of the heat shock response. |
| 巻・号 | 88(23) |
| ページ | 10515-9 |
| 公開日 | 1991-12-1 |
| DOI | 10.1073/pnas.88.23.10515 |
| PMID | 1961716 |
| PMC | PMC52959 |
| MeSH | Base Sequence Chromosome Deletion DNA-Directed RNA Polymerases / genetics DNA-Directed RNA Polymerases / metabolism Escherichia coli / genetics* Gene Expression Regulation, Bacterial* Genes, Bacterial* Heat-Shock Proteins / biosynthesis Heat-Shock Proteins / genetics* Hot Temperature Kinetics Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Protein Biosynthesis* RNA, Messenger / genetics* Recombinant Fusion Proteins / biosynthesis Restriction Mapping Sigma Factor / biosynthesis Sigma Factor / genetics* Software Transcription Factors* beta-Galactosidase / biosynthesis beta-Galactosidase / genetics |
| IF | 9.412 |
| 引用数 | 130 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7975 ME7973 ME7974 |