RRC ID 29805
Author Kato J, Nishimura Y, Suzuki H.
Title Escherichia coli parA is an allele of the gyrB gene.
Journal Mol Gen Genet
Abstract A thermosensitive (ts) parA mutant, MFT110, of Escherichia coli carried at least two ts mutations. The major ts defect, resulting from a mutation mapped originally at 95 min and complemented by pLC8-47, was most probably due to psd. A plasmid carrying the 1.6 kb BamHI-PvuII fragment recloned from pLC8-47 complemented the major ts mutation in MFT110 and psd(ts) in two mutants, but did not correct the Par phenotype of MFT110. The second ts mutation was salt-repairable and mapped at 83 min close to recF and tnaA. This mutation was linked with the Par phenotype as shown unambiguously by 4',6-diamidino-2-phenylindole stained nucleoids in parA mutant cells with the W3110 genetic background. Both salt-repairable ts and Par traits were corrected concomitantly by a plasmid carrying the chromosomal region solely for the gyrB gene. This strongly suggests that parA is an allele of gyrB.
Volume 217(1)
Pages 178-81
Published 1989-5-1
DOI 10.1007/BF00330959
PMID 2549366
MeSH Alleles* Bacterial Proteins / genetics Chromosome Mapping DNA Transposable Elements DNA, Bacterial / biosynthesis DNA, Bacterial / genetics Escherichia coli / genetics* Genes, Bacterial* Genetic Complementation Test Hot Temperature Mutation* Phenotype Plasmids
Times Cited 48
Prokaryotes E. coli ME9028