論文 - 詳細
| RRC ID | 29827 |
|---|---|
| 著者 | Marsh L, Walker GC. |
| タイトル | Cold sensitivity induced by overproduction of UmuDC in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
The UmuD and UmuC proteins of Escherichia coli are essential for mutagenesis by UV and most chemicals. Their mode of action is presently unknown. Strains which lack the LexA repressor [lexA(Def)] and contain a pBR322-derived plasmid carrying the umuDC operon overexpress UmuD and UmuC and become cold sensitive (growth at 42 degrees C but not at 30 degrees C). Deletion mapping showed that the umuDC locus on the plasmid is responsible for conferring cold sensitivity. The conditional lethality appeared due to a rapid and reversible inhibition of DNA synthesis at the nonpermissive temperature. Cold sensitivity was enhanced by the increase of NaCl in the medium to 1% and eliminated by 4% ethanol in the medium. Cold sensitivity was partially suppressed by the lon-100 mutation and completely suppressed by the htpR165 mutation. |
| 巻・号 | 162(1) |
| ページ | 155-61 |
| 公開日 | 1985-4-1 |
| DOI | 10.1128/jb.162.1.155-161.1985 |
| PMID | 2984171 |
| PMC | PMC218968 |
| MeSH | Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Cold Temperature* DNA, Bacterial / biosynthesis Escherichia coli / genetics* Escherichia coli / growth & development Mutation* |
| IF | 3.006 |
| 引用数 | 106 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME8384 ME8383 |