RRC ID 29828
Author Yanisch-Perron C, Vieira J, Messing J.
Title Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
Journal Gene
Abstract Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII.
Volume 33(1)
Pages 103-19
Published 1985
PII 0378-1119(85)90120-9
PMID 2985470
MeSH Base Sequence Cloning, Molecular* Coliphages / genetics* Conjugation, Genetic DNA Restriction Enzymes / metabolism DNA, Single-Stranded / genetics Escherichia coli / metabolism Genetic Vectors* Methylation Mutation Plasmids Recombination, Genetic
IF 2.498
Times Cited 14478
Prokaryotes E. coli ME8649 ME9041 ME8572 pUC18 pUC19