| RRC ID |
29839
|
| 著者 |
Attfield PV, Benson FE, Lloyd RG.
|
| タイトル |
Analysis of the ruv locus of Escherichia coli K-12 and identification of the gene product.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
The ruv gene of Escherichia coli, which is associated with inducible mechanisms of DNA repair and recombination, has been cloned into the low-copy plasmid vector pHSG415. The recombinant plasmid pPVA101 fully complements the DNA repair-deficient phenotype of ruv mutants. Restriction endonuclease analysis of this plasmid revealed a 10.6-kilobase (kb) HindIII DNA insert which contained a 7.7-kb PstI fragment identified as being from the chromosomal ruv region. Deletion analysis and Tn1000 insertional inactivation of ruv function located the ruv coding region to a 2.2-kb section of the cloned DNA fragment. A comparison of the proteins encoded by ruv wild-type and mutant plasmids identified the gene product as a protein of molecular weight 41,000.
|
| 巻・号 |
164(1)
|
| ページ |
276-81
|
| 公開日 |
1985-10-1
|
| DOI |
10.1128/jb.164.1.276-281.1985
|
| PMID |
2995311
|
| PMC |
PMC214240
|
| MeSH |
Bacterial Proteins / analysis*
Chromosome Deletion
Chromosome Mapping*
DNA Repair*
DNA Transposable Elements
Escherichia coli / genetics*
Mutation
Ultraviolet Rays
|
| IF |
3.006
|
| 引用数 |
30
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
ME8575
ME8576 |
