RRC ID 29865
著者 Meier U, Mayer H.
タイトル Genetic location of genes encoding enterobacterial common antigen.
ジャーナル J Bacteriol
Abstract A new rff mutation (rff-726) of Escherichia coli is described which affects the biosynthesis of the enterobacterial common antigen. This mutation was detected in an rfe-defective strain. A Tn10 insertion near the rfe locus was isolated to facilitate further mapping. Both mutations rfe and rff were mapped by transduction with bacteriophage P1, giving the gene order ilv rfe rff uvrD metE. The F' factor F14 was able to complement both mutations rfe and rff, whereas the F' factor F16 could complement the rfe but not the rff mutation. The rff mutation did not affect the biosynthesis of N-acetyl-D-mannosaminuronic acid, as the previously described rff mutations in Salmonella typhimurium do (H. C. Lew, H. Nikaido, and P. H. Mäkelä, J. Bacteriol. 136:227-233, 1978), and also did not affect the biosynthesis of other enterobacterial common antigen components; however, the biosynthesis of the complete enterobacterial common antigen molecule was blocked.
巻・号 163(2)
ページ 756-62
公開日 1985-8-1
DOI 10.1128/jb.163.2.756-762.1985
PMID 3894334
PMC PMC219186
MeSH Antigens, Bacterial / genetics* Coliphages / genetics Crosses, Genetic Escherichia coli / genetics* Escherichia coli / immunology Genes* Genes, Bacterial* Genotype Mutation Species Specificity Transduction, Genetic
IF 3.006
引用数 39
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8488