RRC ID 29891
Author Young IG, Leppik RA, Hamilton JA, Gibson F.
Title Biochemical and genetic studies on ubiquinone biosynthesis in Escherichia coli K-12:4-hydroxybenzoate octaprenyltransferase.
Journal J Bacteriol
Abstract Three ubiquinone-deficient mutants of Escherichia coli unable to convert 4-hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate were isolated and examined. The results of genetic analysis suggest that each of the mutants carries a mutation in a gene designated ubiA which can be represented at minute 79 on the E. coli chromosome map. The conversion of 4-hydroxybenzoate into 3-octaprenyl-4-hydroxybenzoate, catalyzed by 4-hydroxybenzoate octaprenyltransferase, was studied with a strain of E. coli that is blocked in the common pathway of aromatic biosynthesis and consequently accumulates the precursor of the side chain of ubiquinone. Both the side-chain precursor and 4-hydroxybenzoate octaprenyltransferase were shown to be membrane-bound. The enzyme required Mg(2+) for optimal activity. The ubiA(-) mutants were found to lack 4-hydroxybenozate octaprenyltransferase activity, which suggested that the ubiA gene is the structural gene coding for this enzyme.
Volume 110(1)
Pages 18-25
Published 1972-4-1
DOI 10.1128/JB.110.1.18-25.1972
PMID 4552989
PMC PMC247373
MeSH Alkyl and Aryl Transferases Benzoates / pharmacology Carbon Isotopes Chromatography, Thin Layer Chromosome Mapping Coliphages Enzyme Activation Escherichia coli / drug effects Escherichia coli / enzymology* Escherichia coli / growth & development Genes Genetics, Microbial Kinetics Magnesium / pharmacology Mutation Subcellular Fractions / drug effects Subcellular Fractions / enzymology Terpenes Transduction, Genetic Transferases* Ubiquinone / biosynthesis*
IF 3.234
Resource
Prokaryotes E. coli ME5454