RRC ID 29905
Author Silverman M, Simon M.
Title Genetic analysis of flagellar mutants in Escherichia coli.
Journal J Bacteriol
Abstract Flagellar mutants in Escherichia coli were obtained by selection for resistance to the flagellotropic phage chi. F elements covering various regions of the E. coli genome were then constructed, and, on the basis of the ability of these elements to restore flagellar function, the mutations were assigned to three regions of the E. coli chromosome. Region I is between trp and gal; region II is between uvrC and aroD; and region III is between his and uvrC. F elements carrying flagellar mutations were constructed. Stable merodiploid strains with a flagellar defect on the exogenote and another on the endogenote were then prepared. These merodiploids yielded information on the complementation behavior of mutations in a given region. Region III was shown to include at least six cistrons, A, B, C, D, E, and F. Region II was shown to include at least four cistrons, G, H, I, and J. Examination of the phenotypes of the mutants revealed that those with lesions in cistron E of region III produce "polyhooks" and lesions in cistron F of region III result in loss of ability to produce flagellin. Mutants with lesions in cistron J of region II were entirely paralyzed (mot) mutants. Genetic analysis of flagellar mutations in region III suggested that the mutations located in cistrons A, B, C, and E are closely linked and mutations in cistrons D and F are closely linked.
Volume 113(1)
Pages 105-13
Published 1973-1-1
DOI 10.1128/JB.113.1.105-113.1973
PMID 4567134
PMC PMC251608
MeSH Antigens, Bacterial Bacterial Proteins / biosynthesis Chromosome Mapping* Chromosomes, Bacterial Coliphages Diploidy Escherichia coli / cytology* Escherichia coli / immunology Escherichia coli / metabolism Flagella* / immunology Flagella* / metabolism Genetic Complementation Test Genetic Techniques Lysogeny Microscopy, Electron Mutation* Phenotype Recombination, Genetic Transduction, Genetic
IF 3.006
Resource
Prokaryotes E. coli ME8053 ME8054 ME8056