Reference - Detail
| RRC ID | 29914 |
|---|---|
| Author | Clarke SJ, Low B, Konigsberg WH. |
| Title | Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12. |
| Journal | J Bacteriol |
| Abstract |
Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA. |
| Volume | 113(3) |
| Pages | 1091-5 |
| Published | 1973-3-1 |
| DOI | 10.1128/jb.113.3.1091-1095.1973 |
| PMID | 4570768 |
| PMC | PMC251668 |
| MeSH | Amino Acyl-tRNA Synthetases / biosynthesis* Amino Acyl-tRNA Synthetases / metabolism Carbon Isotopes Cell-Free System Chromosome Mapping Crosses, Genetic Escherichia coli / enzymology* Escherichia coli / growth & development Escherichia coli / metabolism Genes* Genetic Linkage* Mutagens Mutation Nitrosoguanidines Pyruvates Recombination, Genetic Serine / metabolism Temperature Transaminases / biosynthesis* Transaminases / metabolism Transduction, Genetic |
| IF | 3.006 |
| Resource | |
| Prokaryotes E. coli | ME8695 |