RRC ID |
29927
|
Author |
Lloyd RG, Low B, Godson GN, Birge EA.
|
Title |
Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype.
|
Journal |
J Bacteriol
|
Abstract |
A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.
|
Volume |
120(1)
|
Pages |
407-15
|
Published |
1974-10-1
|
DOI |
10.1128/jb.120.1.407-415.1974
|
PMID |
4607624
|
PMC |
PMC245777
|
MeSH |
Alleles
Cell Survival
Chromosome Mapping
Conjugation, Genetic
DNA Nucleotidyltransferases / biosynthesis
DNA, Bacterial / biosynthesis
Escherichia coli* / enzymology
Escherichia coli* / growth & development
Escherichia coli* / isolation & purification
Escherichia coli* / metabolism
Escherichia coli* / radiation effects
Genes
Genetic Complementation Test
Genetic Linkage
Mutagens
Mutation*
Nitrosoguanidines
Phenotype
Radiation Effects
Recombination, Genetic*
Temperature
Thymidine / metabolism
Transduction, Genetic
Tritium
Ultraviolet Rays
|
IF |
3.006
|
Resource |
Prokaryotes E. coli |
ME5401
ME7101 |