| RRC ID |
29961
|
| 著者 |
Hane MW, Wood TH.
|
| タイトル |
Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.
|
| ジャーナル |
J Bacteriol
|
| Abstract |
Escherichia coli K-12 strains tested so far (approximately 20) can be separated into three groups on the basis of their abilities to form colonies on nutrient agar supplemented with nalidixic acid (NAL): (i) Nal(s) or wild type (no growth at 1 to 2 mug/ml); (ii) NalA(r) (growth at 40 mug/ml or higher); and (iii) NalB(r) (growth at 4 mug/ml, but no growth at 10 mug/ml). The NalA(r) group has a spectrum of sensitivity ranging from 60 to over 100 mug/ml. All Hfr strains of the NalA(r) and NalB(r) groups transfer NAL resistance to recipient cells at genetic loci which are at 42.5 +/- 0.5 and 51 +/- 1 min, respectively, on the Taylor-Trotter map. Some members of the NalA(r) group also have the genetic locus for NalB(r). The nalA(s) allele is completely dominant to nalA(r) in a partial diploid configuration. In haploids, nalA(r)-nalB(r) is phenotypically NalA(r); nalA(r)-nalB(s) is NalA(r); and nalA(s)-nalB(r) is NalB(r). The map location of nalA and the easy differentiation between NalA(r) and NalA(s) allow this marker to be used as a counterselector in bacterial conjugation experiments.
|
| 巻・号 |
99(1)
|
| ページ |
238-41
|
| 公開日 |
1969-7-1
|
| DOI |
10.1128/jb.99.1.238-241.1969
|
| PMID |
4895844
|
| PMC |
PMC249993
|
| MeSH |
Alleles
Chromosome Mapping
Conjugation, Genetic
Drug Resistance, Microbial*
Escherichia coli / drug effects*
Genes, Dominant
Genetics, Microbial*
Mutation
Nalidixic Acid / pharmacology*
|
| IF |
3.006
|
| リソース情報 |
| 原核生物(大腸菌) |
ME5471 |
