論文 - 詳細
| RRC ID | 29961 |
|---|---|
| 著者 | Hane MW, Wood TH. |
| タイトル | Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. |
| ジャーナル | J Bacteriol |
| Abstract |
Escherichia coli K-12 strains tested so far (approximately 20) can be separated into three groups on the basis of their abilities to form colonies on nutrient agar supplemented with nalidixic acid (NAL): (i) Nal(s) or wild type (no growth at 1 to 2 mug/ml); (ii) NalA(r) (growth at 40 mug/ml or higher); and (iii) NalB(r) (growth at 4 mug/ml, but no growth at 10 mug/ml). The NalA(r) group has a spectrum of sensitivity ranging from 60 to over 100 mug/ml. All Hfr strains of the NalA(r) and NalB(r) groups transfer NAL resistance to recipient cells at genetic loci which are at 42.5 +/- 0.5 and 51 +/- 1 min, respectively, on the Taylor-Trotter map. Some members of the NalA(r) group also have the genetic locus for NalB(r). The nalA(s) allele is completely dominant to nalA(r) in a partial diploid configuration. In haploids, nalA(r)-nalB(r) is phenotypically NalA(r); nalA(r)-nalB(s) is NalA(r); and nalA(s)-nalB(r) is NalB(r). The map location of nalA and the easy differentiation between NalA(r) and NalA(s) allow this marker to be used as a counterselector in bacterial conjugation experiments. |
| 巻・号 | 99(1) |
| ページ | 238-41 |
| 公開日 | 1969-7-1 |
| DOI | 10.1128/jb.99.1.238-241.1969 |
| PMID | 4895844 |
| PMC | PMC249993 |
| MeSH | Alleles Chromosome Mapping Conjugation, Genetic Drug Resistance, Microbial* Escherichia coli / drug effects* Genes, Dominant Genetics, Microbial* Mutation Nalidixic Acid / pharmacology* |
| IF | 3.006 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME5471 |