論文 - 詳細
| RRC ID | 29993 |
|---|---|
| 著者 | Lennette ET, Apirion D. |
| タイトル | Genetic analysis of an Escherichia coli syndrome. |
| ジャーナル | J Bacteriol |
| Abstract |
A mutant strain of Escherichia coli that fails to recover from prolonged (72 hr) starvation also fails to grow at 43 C. Extracts of this mutant strain show an increased ribonuclease II activity as compared to extracts of the parental strain, and stable ribonucleic acid is degraded to a larger extent in this strain during starvation. Ts(+) transductants and revertants were tested for all the above-mentioned phenotypes. All the Ts(+) transductants and revertants tested behaved like the Ts(+) parental strain, which suggests that all the observed phenotypes are caused by a single sts (starvation-temperature sensitivity) mutation. The reversion rate from sts(-) to sts(+) is rather low but is within the range of reversion rates for other single-site mutations. Three-point transduction crosses located this sts mutation between the ilv and rbs genes. The properties of sts(+)/sts(-) merozygotes suggested that the Ts(-) phenotype of this mutation is recessive. |
| 巻・号 | 108(3) |
| ページ | 1322-8 |
| 公開日 | 1971-12-1 |
| DOI | 10.1128/jb.108.3.1322-1328.1971 |
| PMID | 4945197 |
| PMC | PMC247222 |
| MeSH | Carbon Isotopes Cell-Free System Chromosome Mapping Conjugation, Genetic Culture Media Cysteine / metabolism Escherichia coli / enzymology Escherichia coli / growth & development Escherichia coli / metabolism* Genes, Recessive Genetics, Microbial* Glycerol / metabolism Hot Temperature* Leucine / metabolism Mutation* Nucleic Acid Denaturation Phenotype RNA, Bacterial / metabolism Recombination, Genetic Ribonucleases / metabolism Transduction, Genetic Tritium Ultrasonics Uracil / metabolism Uracil Nucleotides / metabolism Vibration |
| IF | 3.006 |
| リソース情報 | |
| 原核生物(大腸菌) | ME6177 ME6178 |