RRC ID 30017
Author Tobe T, Ito K, Yura T.
Title Isolation and physical mapping of temperature-sensitive mutants defective in heat-shock induction of proteins in Escherichia coli.
Journal Mol. Gen. Genet.
Abstract Mutants of Escherichia coli K12 that are partially or totally defective in induction of major heat-shock proteins and cannot grow at high temperature (42 degrees C) were isolated by localized mutagenesis. These mutants carry a single mutation in the gene htp R (formerly hin) located at min 76 on the E. coli genetic map. Some mutants exhibit delayed (partial) induction of heat-shock proteins or require a higher temperature for induction than the wild type, whereas others are not induced under any of these conditions. The maximum temperature that allows growth varies among different mutants and is correlated with the residual induction capacity. Temperature-resistant revertants obtained from each mutant are fully or partially recovered in heat-shock induction. These results indicate that the inability of htp R mutants to grow at high temperature is due to the defect in heat-shock induction. In addition, a couple of mutants was found that produce significantly higher amounts of heat-shock proteins even at 30 degrees C. The htp R gene has been cloned into plasmid pBR322 using the above mutants, and was localized to a DNA segment of 1.6 kilobase pairs. The mutants harboring certain plasmids that carry a part of htp R produce temperature-resistant recombinants at high frequency. This permits further localization of mutations within the htp R gene. Analysis of proteins encoded by each of the recombinant plasmids including the one carrying a previously isolated amber mutation (htp R165) led to the identification of a protein with an apparent molecular weight of about 36,000 daltons as the htp R gene product.
Volume 195(1-2)
Pages 10-6
Published 1984
PMID 6092838
MeSH Bacterial Proteins / isolation & purification Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant / metabolism Escherichia coli / genetics* Escherichia coli / isolation & purification Genes* Genes, Bacterial* Genotype Heat-Shock Proteins / genetics* Hot Temperature Molecular Weight Mutation* Plasmids Species Specificity Temperature
Times Cited 82
Prokaryotes E. coli ME7984 ME7942 ME7943 ME7942 ME7943 ME7977 ME7938 ME7939 ME7941 ME7944 ME7929 ME7976 ME7971 ME7972 ME7946 ME7947 ME7933 ME7934 ME7936 ME7937 ME7940 ME7930 ME7931 ME7932 ME7935