RRC ID 30053
Author Brooks JE, Blumenthal RM, Gingeras TR.
Title The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
Journal Nucleic Acids Res
Abstract The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms.
Volume 11(3)
Pages 837-51
Published 1983-2-11
DOI 10.1093/nar/11.3.837
PMID 6300769
PMC PMC325756
MeSH Amino Acid Sequence Base Sequence Cloning, Molecular* DNA (Cytosine-5-)-Methyltransferases / genetics* DNA Restriction Enzymes DNA, Bacterial / genetics DNA, Bacterial / isolation & purification* Escherichia coli / enzymology* Genes* Genes, Bacterial* Methyltransferases / genetics* Nucleic Acid Hybridization Plasmids
IF 11.147
Times Cited 152
Prokaryotes E. coli ME9000