RRC ID 30078
Author Chang YY, Cronan JE Jr.
Title Genetic and biochemical analyses of Escherichia coli strains having a mutation in the structural gene (poxB) for pyruvate oxidase.
Journal J Bacteriol
Abstract Mutants of Escherichia coli K-12 deficient in pyruvate oxidase were isolated from an aceEF (pyruvate dehydrogenase-deficient) strain by selection for a complete absence of growth on medium lacking acetate. Extracts of two of the mutants were shown to contain normal levels of pyruvate oxidase antigen, although the enzymatic activities of the extracts were reduced or absent. The poxB locus was mapped by using closely linked transposon insertions to min 18.7 of the E. coli linkage map between the cmlA and aroA loci, a location far removed from that of the regulatory gene, poxA.
Volume 154(2)
Pages 756-62
Published 1983-5-1
DOI 10.1128/jb.154.2.756-762.1983
PMID 6341362
PMC PMC217526
MeSH Chromosome Mapping Chromosomes, Bacterial Escherichia coli / enzymology Escherichia coli / genetics* Genes* Genes, Bacterial Genes, Regulator Genetic Linkage Mutation Pyruvate Oxidase / genetics* Pyruvate Oxidase / metabolism
IF 3.006
Times Cited 51
WOS Category MICROBIOLOGY
Resource
Prokaryotes E. coli ME8428 ME8433 ME8491 ME8489 ME8490