Reference - Detail
| RRC ID | 30108 |
|---|---|
| Author | Oliver DB, Beckwith J. |
| Title | E. coli mutant pleiotropically defective in the export of secreted proteins. |
| Journal | Cell |
| Abstract |
A hybrid beta-galactosidase molecule containing a substantial portion of the amino-terminal sequence of the maltose-binding protein is inserted in the cytoplasmic membrane of E. coli; in this location, the protein has very low enzymatic activity. The strain producing it is, therefore, Lac-. Selection for derivatives of the fusion strain that are able to grow on lactose yields mutants in which the hybrid protein has become cytoplasmic, and thus has higher enzymatic activity. Among such derivatives, we have isolated a temperature-sensitive conditional lethal mutant that accumulates the precursor of the maltose-binding protein in the cytoplasm, and also accumulates precursors of alkaline phosphatase, lambda receptor protein and the ompF gene gene product. A number of periplasmic proteins are, however, properly localized at the nonpermissive temperature. The temperature-sensitive lesion has been genetically mapped to 2.5 min on the E. coli map, within or near a cluster of genes responsible for cell division and septation. The principle behind the genetic selection employed here should be useful in obtaining other secretion mutants to characterize the cell's secretion machinery. |
| Volume | 25(3) |
| Pages | 765-72 |
| Published | 1981-9-1 |
| DOI | 10.1016/0092-8674(81)90184-7 |
| PII | 0092-8674(81)90184-7 |
| PMID | 7026050 |
| MeSH | ATP-Binding Cassette Transporters* Bacterial Proteins / metabolism* Carrier Proteins / metabolism Cell Compartmentation Cell Membrane / metabolism Chromosome Mapping Chromosomes, Bacterial / ultrastructure Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli / ultrastructure Escherichia coli Proteins* Maltose / metabolism Maltose-Binding Proteins Membrane Proteins / metabolism Monosaccharide Transport Proteins* Mutation Periplasmic Binding Proteins* |
| IF | 38.637 |
| Times Cited | 443 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | ME8988 |