RRC ID 30137
Author Yamanaka K, Ogura T, Niki H, Hiraga S.
Title Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli.
Journal Mol Gen Genet
Abstract We have previously reported that the MukB protein is essential for chromosome partitioning in Escherichia coli and that mukB mutants produce anucleate cells and are temperature-sensitive for colony formation. The mukB gene maps at 21 min on the E. coli chromosome and smtA-mukF-mukE-mukB genes might comprise an operon, which is transcribed in a clockwise direction. Here, we report that mukF and mukE null mutants are both temperature-sensitive for colony formation and produce anucleate cells even at the permissive temperature. These phenotypes are the same as those observed in the mukB null mutant. The primary sequence of MukF includes a leucine zipper structure and an acidic domain. Mutational analysis revealed that both are required for MukF function. When the MukF protein was overproduced in the wild-type strain, anucleate cells were produced. In contrast, overproduction of either MukE or MukB did not cause the defect. In null mutants for the mukF, mukE, and mukB genes, the synchronous initiation of chromosome replication was not affected. The mini-F plasmid was as stably maintained in these mutants as in the wild-type strain. These results indicate that the MukF, MukE, and MukB proteins are involved in the chromosome partitioning steps, but are not required for mini-F plasmid partitioning.
Volume 250(3)
Pages 241-51
Published 1996-2-25
DOI 10.1007/BF02174381
PMID 8602138
MeSH Amino Acid Sequence Bacterial Proteins / chemistry Bacterial Proteins / genetics* Bacterial Proteins / physiology Cell Division / genetics Cell Nucleus / genetics Cell Nucleus / metabolism Chromosomal Proteins, Non-Histone* Chromosomes, Bacterial / metabolism* DNA Mutational Analysis Escherichia coli / chemistry Escherichia coli / genetics* Escherichia coli / growth & development Escherichia coli / ultrastructure Escherichia coli Proteins* Flow Cytometry Fluorescent Dyes / metabolism Gene Expression Regulation, Bacterial Genes, Bacterial / genetics* Indoles / metabolism Leucine Zippers Molecular Sequence Data Mutation Phenotype Plasmids / genetics Plasmids / metabolism Repressor Proteins* Temperature
Times Cited 107
Prokaryotes E. coli ME7828 ME7927 ME7928 ME7816 ME7817 ME7818 ME7819 ME7820 ME7796 ME7797 ME7798