論文 - 詳細
| RRC ID | 30139 |
|---|---|
| 著者 | Kogoma T, Cadwell GW, Barnard KG, Asai T. |
| タイトル | The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair. |
| ジャーナル | J Bacteriol |
| Abstract |
The PriA protein, a component of the phiX174-type primosome, was previously shown to be essential for damage-inducible DNA replication in Escherichia coli, termed inducible stable DNA replication. Here, we show that priA::kan null mutants are defective in transductional and conjugational homologous recombination and are hypersensitive to mitomycin C and gamma rays, which cause double-strand breaks. The introduction of a plasmid carrying the priA300 allele, which encodes a mutant PriA protein capable of catalyzing the assembly of an active primosome but which is missing the n'-pas-dependent ATPase, helicase, and translocase activities associated with PriA, alleviates the defects of priA::kan mutants in homologous recombination, double-strand break repair, and inducible stable DNA replication. Furthermore, spa-47, which was isolated as a suppressor of the broth sensitivity of priA::kan mutants, suppresses the Rec- and mitomycin C sensitivity phenotypes of priA::kan mutants. The spa-47 suppressor mutation maps within or very near dnaC. These results suggest that PriA-dependent primosome assembly is crucial for both homologous recombination and double-strand break repair and support the proposal that these processes in E. coli involve extensive DNA replication. |
| 巻・号 | 178(5) |
| ページ | 1258-64 |
| 公開日 | 1996-3-1 |
| DOI | 10.1128/jb.178.5.1258-1264.1996 |
| PMID | 8631700 |
| PMC | PMC177797 |
| MeSH | Bacterial Proteins / metabolism DNA Damage DNA Repair* DNA Replication* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Dose-Response Relationship, Radiation Escherichia coli / genetics* Escherichia coli Proteins* Exodeoxyribonucleases / metabolism Mutation Recombination, Genetic* Replication Protein A Suppression, Genetic Ultraviolet Rays / adverse effects |
| IF | 3.006 |
| 引用数 | 147 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9579 ME9422 ME9376 ME9590 ME9591 ME9561 ME9530 ME9593 |