論文 - 詳細
| RRC ID | 30147 |
|---|---|
| 著者 | Kanemori M, Nishihara K, Yanagi H, Yura T. |
| タイトル | Synergistic roles of HslVU and other ATP-dependent proteases in controlling in vivo turnover of sigma32 and abnormal proteins in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Production of abnormal proteins during steady-state growth induces the heat shock response by stabilizing normally unstable sigma32 (encoded by the rpoH gene) specifically required for transcription of heat shock genes. We report here that a multicopy plasmid carrying the hslVU operon encoding a novel ATP-dependent protease inhibits the heat shock response induced by production of human prourokinase (proUK) in Escherichia coli. The overproduction of HslVU (ClpQY) protease markedly reduced the stability and accumulation of proUK and thus reduced the induction of heat shock proteins. In agreement with this finding, deletion of the chromosomal hslVU genes significantly enhanced levels of proUK and sigma32 without appreciably affecting cell growth. When the deltahslVU deletion was combined with another protease mutation (lon, clpP, or ftsH/hflB), the resulting multiple mutations caused higher stabilization of proUK and sigma32, enhanced synthesis of heat shock proteins, and temperature-sensitive growth. Furthermore, overproduction of HslVU protease reduced sigma32 levels in strains that were otherwise expected to produce enhanced levels of sigma32 due either to the absence of Lon-ClpXP proteases or to the limiting levels of FtsH protease. Thus, a set of ATP-dependent proteases appear to play synergistic roles in the negative control of the heat shock response by modulating in vivo turnover of sigma32 as well as through degradation of abnormal proteins. |
| 巻・号 | 179(23) |
| ページ | 7219-25 |
| 公開日 | 1997-12-1 |
| DOI | 10.1128/jb.179.23.7219-7225.1997 |
| PMID | 9393683 |
| PMC | PMC179669 |
| MeSH | ATP-Dependent Proteases Adenosine Triphosphatases / metabolism* Bacterial Proteins / metabolism Endopeptidase Clp Endopeptidases / metabolism* Escherichia coli / enzymology* Escherichia coli Proteins* Gene Deletion Genes, Bacterial Heat-Shock Proteins / genetics Heat-Shock Proteins / metabolism* Heat-Shock Response / genetics* Humans Membrane Proteins / metabolism Mutation Protease La* Recombinant Proteins / genetics Recombinant Proteins / metabolism Serine Endopeptidases / genetics Serine Endopeptidases / metabolism Sigma Factor / metabolism* Transcription Factors* Urokinase-Type Plasminogen Activator / genetics Urokinase-Type Plasminogen Activator / metabolism* |
| IF | 3.006 |
| 引用数 | 138 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME7968 |