論文 - 詳細
| RRC ID | 30148 |
|---|---|
| 著者 | Murphy KC. |
| タイトル | Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable. |
| 巻・号 | 180(8) |
| ページ | 2063-71 |
| 公開日 | 1998-4-1 |
| DOI | 10.1128/JB.180.8.2063-2071.1998 |
| PMID | 9555887 |
| PMC | PMC107131 |
| MeSH | Bacteriophage lambda / genetics* Calcium / pharmacology Chromosomes, Bacterial / genetics* Cloning, Molecular / methods DNA Primers DNA, Superhelical Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / virology Gene Targeting / methods* Genotype Operon Plasmids Polymerase Chain Reaction Recombinant Fusion Proteins / biosynthesis Recombination, Genetic* Restriction Mapping |
| IF | 3.006 |
| 引用数 | 367 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME5076 |