Reference - Detail
| RRC ID | 30148 |
|---|---|
| Author | Murphy KC. |
| Title | Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. |
| Journal | J Bacteriol |
| Abstract |
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable. |
| Volume | 180(8) |
| Pages | 2063-71 |
| Published | 1998-4-1 |
| DOI | 10.1128/JB.180.8.2063-2071.1998 |
| PMID | 9555887 |
| PMC | PMC107131 |
| MeSH | Bacteriophage lambda / genetics* Calcium / pharmacology Chromosomes, Bacterial / genetics* Cloning, Molecular / methods DNA Primers DNA, Superhelical Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / virology Gene Targeting / methods* Genotype Operon Plasmids Polymerase Chain Reaction Recombinant Fusion Proteins / biosynthesis Recombination, Genetic* Restriction Mapping |
| IF | 3.006 |
| Times Cited | 367 |
| WOS Category | MICROBIOLOGY |
| Resource | |
| Prokaryotes E. coli | ME5076 |