RRC ID 30148
Author Murphy KC.
Title Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.
Journal J Bacteriol
Abstract Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.
Volume 180(8)
Pages 2063-71
Published 1998-4-1
DOI 10.1128/JB.180.8.2063-2071.1998
PMID 9555887
PMC PMC107131
MeSH Bacteriophage lambda / genetics* Calcium / pharmacology Chromosomes, Bacterial / genetics* Cloning, Molecular / methods DNA Primers DNA, Superhelical Escherichia coli / drug effects Escherichia coli / genetics* Escherichia coli / virology Gene Targeting / methods* Genotype Operon Plasmids Polymerase Chain Reaction Recombinant Fusion Proteins / biosynthesis Recombination, Genetic* Restriction Mapping
IF 3.006
Times Cited 367
Prokaryotes E. coli ME5076