RRC ID 30152
Author Nakano H, Yamada Y, Ishikura H, Inokuchi H.
Title A mutation in the gene for trigger factor suppresses the defect in cell division in the divE42 mutant of Escherichia coli K12.
Journal Mol Gen Genet
Abstract A total of sixteen spontaneously generated, independent suppressor mutants was isolated from a mutant (divE42) of Escherichia coli K12 that is defective in cell division. One of the suppressor mutants, designated TR4, had a novel phenotype: it was able to grow at 42 degrees C but not at 32 degrees C. The Kohara genomic library was screened for complementing clones. Clone 148 was able to complement the mutation responsible for the cold-sensitive phenotype, and the gene for trigger factor (tig), which encodes a ribosome-associated peptidyl-prolyl cis/trans isomerase, was identified as the mutated gene by deletion analysis with the insert DNA from clone 148. DNA sequencing revealed that the mutation in the tig gene of the TR4 suppressor mutant was a single nucleotide insertion (+A) at a distance of 834 nucleotides from the initiation codon for this enzyme. When the wild-type tig gene was introduced into the TR4 suppressor mutant, the bacteria were able to grow at 32 degrees C but not at 42 degrees C, an indication that the intergenic suppressor mutation was recessive to the wild-type allele. A model is proposed that accounts for the phenotypes of the divE42 mutant and the TR4 suppressor mutant.
Volume 260(1)
Pages 75-80
Published 1998-10-1
DOI 10.1007/s004380050872
PMID 9829830
MeSH Cell Division / genetics Escherichia coli / cytology* Escherichia coli / genetics* Gene Expression Genes, Bacterial Genetic Complementation Test Models, Genetic Mutation* Peptidylprolyl Isomerase / genetics* RNA, Transfer, Ser / genetics Sequence Analysis, DNA Sequence Deletion Suppression, Genetic* Temperature beta-Galactosidase / biosynthesis
Times Cited 2
Prokaryotes E. coli IH46