論文 - 詳細
| RRC ID | 30181 |
|---|---|
| 著者 | Yoshida K, Ohki YH, Murata M, Kinehara M, Matsuoka H, Satomura T, Ohki R, Kumano M, Yamane K, Fujita Y. |
| タイトル | Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH. |
| ジャーナル | J Bacteriol |
| Abstract |
The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA is a repressor of its own operon, while LmrB acts as a multidrug efflux transporter. LmrA was produced in Escherichia coli cells and was shown to bind to the lmr promoter region, in which an LmrA-binding site was identified. Genome-wide screening involving DNA microarray analysis allowed us to conclude that LmrA also repressed yxaGH, which was not likely to contribute to the multidrug resistance. LmrA bound to a putative yxaGH promoter region, in which two tandem LmrA-binding sites were identified. The LmrA regulon was thus determined to comprise lmrAB and yxaGH. All three LmrA-binding sites contained an 18-bp consensus sequence, TAGACCRKTCWMTATAWT, which could play an important role in LmrA binding. |
| 巻・号 | 186(17) |
| ページ | 5640-8 |
| 公開日 | 2004-9-1 |
| DOI | 10.1128/JB.186.17.5640-5648.2004 |
| PII | 186/17/5640 |
| PMID | 15317768 |
| PMC | PMC516806 |
| MeSH | Amino Acid Sequence Bacillus subtilis / genetics* Bacterial Proteins / genetics Bacterial Proteins / metabolism Bacterial Proteins / physiology Base Sequence Binding Sites Cloning, Molecular Consensus Sequence Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Profiling Gene Expression Regulation, Bacterial* Molecular Sequence Data Multidrug Resistance-Associated Proteins / genetics Multidrug Resistance-Associated Proteins / physiology* Oligonucleotide Array Sequence Analysis Operon Promoter Regions, Genetic Protein Binding Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism Regulon Repressor Proteins / genetics Repressor Proteins / metabolism* Repressor Proteins / physiology* |
| IF | 3.006 |
| 引用数 | 17 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | ME9665 |
| 原核生物(枯草菌) | MBS281 MBS282 MBS283 |