Reference - Detail
| RRC ID | 30237 |
|---|---|
| Author | Miyagawa K, Low RS, Santosa V, Tsuji H, Moser BA, Fujisawa S, Harland JL, Raguimova ON, Go A, Ueno M, Matsuyama A, Yoshida M, Nakamura TM, Tanaka K. |
| Title | SUMOylation regulates telomere length by targeting the shelterin subunit Tpz1(Tpp1) to modulate shelterin-Stn1 interaction in fission yeast. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Telomeres protect DNA ends of linear eukaryotic chromosomes from degradation and fusion, and ensure complete replication of the terminal DNA through recruitment of telomerase. The regulation of telomerase is a critical area of telomere research and includes cis regulation by the shelterin complex in mammals and fission yeast. We have identified a key component of this regulatory pathway as the SUMOylation [the covalent attachment of a small ubiquitin-like modifier (SUMO) to target proteins] of a shelterin subunit in fission yeast. SUMOylation is known to be involved in the negative regulation of telomere extension by telomerase; however, how SUMOylation limits the action of telomerase was unknown until now. We show that SUMOylation of the shelterin subunit TPP1 homolog in Schizosaccharomyces pombe (Tpz1) on lysine 242 is important for telomere length homeostasis. Furthermore, we establish that Tpz1 SUMOylation prevents telomerase accumulation at telomeres by promoting recruitment of Stn1-Ten1 to telomeres. Our findings provide major mechanistic insights into how the SUMOylation pathway collaborates with shelterin and Stn1-Ten1 complexes to regulate telomere length. |
| Volume | 111(16) |
| Pages | 5950-5 |
| Published | 2014-4-22 |
| DOI | 10.1073/pnas.1401359111 |
| PII | 1401359111 |
| PMID | 24711392 |
| PMC | PMC4000806 |
| MeSH | Carrier Proteins / metabolism* DNA-Binding Proteins G2 Phase Ligases Lysine / metabolism Models, Biological Protein Binding Protein Subunits / metabolism* S Phase Schizosaccharomyces / cytology Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* Sumoylation* Telomerase / metabolism Telomere / metabolism* Telomere Homeostasis* Telomere Shortening Telomere-Binding Proteins / metabolism* Ubiquitin-Protein Ligases / metabolism |
| IF | 9.412 |
| Times Cited | 32 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Yeast | FY14150 FY14135 FY14283 FY14283 FY14256 |