| RRC ID |
30240
|
| 著者 |
Ohneda K, Moriguchi T, Ohmori S, Ishijima Y, Satoh H, Philipsen S, Yamamoto M.
|
| タイトル |
Transcription factor GATA1 is dispensable for mast cell differentiation in adult mice.
|
| ジャーナル |
Mol Cell Biol
|
| Abstract |
Although previous studies have shown that GATA1 is required for mast cell differentiation, the effects of the complete ablation of GATA1 in mast cells have not been examined. Using conditional Gata1 knockout mice (Gata1(-/y)), we demonstrate here that the complete ablation of GATA1 has a minimal effect on the number and distribution of peripheral tissue mast cells in adult mice. The Gata1(-/y) bone marrow cells were capable of differentiating into mast cells ex vivo. Microarray analyses showed that the repression of GATA1 in bone marrow mast cells (BMMCs) has a small impact on the mast cell-specific gene expression in most cases. Interestingly, however, the expression levels of mast cell tryptases in the mouse chromosome 17A3.3 were uniformly reduced in the GATA1 knockdown cells, and GATA1 was found to bind to a 500-bp region at the 5' end of this locus. Revealing a sharp contrast to that observed in the Gata1-null BMMCs, GATA2 deficiency resulted in a significant loss of the c-Kit(+) FcεRIα(+) mast cell fraction and a reduced expression of several mast cell-specific genes. Collectively, GATA2 plays a more important role than GATA1 in the regulation of most mast cell-specific genes, while GATA1 might play specific roles in mast cell functions.
|
| 巻・号 |
34(10)
|
| ページ |
1812-26
|
| 公開日 |
2014-5-1
|
| DOI |
10.1128/MCB.01524-13
|
| PII |
MCB.01524-13
|
| PMID |
24615013
|
| PMC |
PMC4019035
|
| MeSH |
Animals
Base Sequence
Bone Marrow Cells / cytology
Bone Marrow Cells / metabolism
Cell Differentiation*
Cells, Cultured
Chromosomes, Mammalian
Culture Media
GATA1 Transcription Factor / physiology*
GATA2 Transcription Factor / physiology*
Gene Expression Regulation, Enzymologic
Male
Mast Cells / physiology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular Sequence Data
Transcriptome
Tryptases / genetics
Tryptases / metabolism
|
| IF |
3.611
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC01888 |
