RRC ID 30249
著者 Ieki T, Okada S, Aihara Y, Ohmoto M, Abe K, Yasuoka A, Misaka T.
タイトル Transgenic labeling of higher order neuronal circuits linked to phospholipase C-β2-expressing taste bud cells in medaka fish.
ジャーナル J Comp Neurol
Abstract The sense of taste plays a pivotal role in the food-selecting behaviors of vertebrates. We have shown that the fish ortholog of the phospholipase C gene (plc-β2) is expressed in a subpopulation of taste bud cells that transmit taste stimuli to the central nervous system to evoke favorable and aversive behaviors. We generated transgenic medaka expressing wheat germ agglutinin (WGA) under the control of a regulatory region of the medaka plc-β2 gene to analyze the neuronal circuit connected to these sensory cells. Immunohistochemical analysis of the transgenic fish 12 days post fertilization revealed that the WGA protein was transferred to cranial sensory ganglia and several nuclei in the hindbrain. WGA signals were also detected in the secondary gustatory nucleus in the hindbrain of 3-month-old transgenic fish. WGA signals were observed in several diencephalic and telencephalic regions in 9-month-old transgenic fish. The age-dependent increase in the labeled brain regions strongly suggests that labeling occurred at taste bud cells and progressively extended to cranial nerves and neurons in the central nervous system. These data are the first to demonstrate the tracing of higher order gustatory neuronal circuitry that is associated with a specific subpopulation of taste bud cells. These results provide insight into the basic neuronal architecture of gustatory information processing that is common among vertebrates.
巻・号 521(8)
ページ 1781-802
公開日 2013-6-1
DOI 10.1002/cne.23256
PMID 23124957
MeSH Age Factors Animals Animals, Genetically Modified Female Gene Expression Regulation, Developmental / genetics Larva Male Nerve Net / metabolism* Neurons / classification Neurons / metabolism* Oryzias / anatomy & histology* Phospholipase C beta / genetics* Phospholipase C beta / metabolism* Taste Buds / cytology* Wheat Germ Agglutinins / genetics Wheat Germ Agglutinins / metabolism
IF 2.801
引用数 2
WOS 分野 ZOOLOGY NEUROSCIENCES
リソース情報
メダカ Cab-Tg(mfplcb2-GFP-WGA) (TG995) OK-Cab (MT830)