論文 - 詳細
| RRC ID | 30260 |
|---|---|
| 著者 | Yoshida Y, Kuroiwa H, Misumi O, Yoshida M, Ohnuma M, Fujiwara T, Yagisawa F, Hirooka S, Imoto Y, Matsushita K, Kawano S, Kuroiwa T. |
| タイトル | Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan. |
| ジャーナル | Science |
| Abstract |
In chloroplast division, the plastid-dividing (PD) ring is a main structure of the PD machinery and is a universal structure in the plant kingdom. However, the components and formation of the PD ring have been enigmatic. By proteomic analysis of PD machineries isolated from Cyanidioschyzon merolae, we identified the glycosyltransferase protein plastid-dividing ring 1 (PDR1), which constructs the PD ring and is widely conserved from red alga to land plants. Electron microscopy showed that the PDR1 protein forms a ring with carbohydrates at the chloroplast-division site. Fluorometric saccharide ingredient analysis of purified PD ring filaments showed that only glucose was included, and down-regulation of PDR1 impaired chloroplast division. Thus, the chloroplasts are divided by the PD ring, which is a bundle of PDR1-mediated polyglucan filaments. |
| 巻・号 | 329(5994) |
| ページ | 949-53 |
| 公開日 | 2010-8-20 |
| DOI | 10.1126/science.1190791 |
| PII | 329/5994/949 |
| PMID | 20724635 |
| MeSH | Algal Proteins / genetics Algal Proteins / isolation & purification Algal Proteins / physiology* Chloroplasts / chemistry Chloroplasts / physiology* Chloroplasts / ultrastructure Cytoskeleton / chemistry Cytoskeleton / physiology* Down-Regulation Glucans / isolation & purification Glucans / physiology* Glycosyltransferases / genetics Glycosyltransferases / isolation & purification Glycosyltransferases / physiology* Protein Binding Proteomics Rhodophyta / genetics Rhodophyta / physiology* Rhodophyta / ultrastructure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 41.846 |
| 引用数 | 55 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-3377 |