RRC ID 30260
著者 Yoshida Y, Kuroiwa H, Misumi O, Yoshida M, Ohnuma M, Fujiwara T, Yagisawa F, Hirooka S, Imoto Y, Matsushita K, Kawano S, Kuroiwa T.
タイトル Chloroplasts divide by contraction of a bundle of nanofilaments consisting of polyglucan.
ジャーナル Science
Abstract In chloroplast division, the plastid-dividing (PD) ring is a main structure of the PD machinery and is a universal structure in the plant kingdom. However, the components and formation of the PD ring have been enigmatic. By proteomic analysis of PD machineries isolated from Cyanidioschyzon merolae, we identified the glycosyltransferase protein plastid-dividing ring 1 (PDR1), which constructs the PD ring and is widely conserved from red alga to land plants. Electron microscopy showed that the PDR1 protein forms a ring with carbohydrates at the chloroplast-division site. Fluorometric saccharide ingredient analysis of purified PD ring filaments showed that only glucose was included, and down-regulation of PDR1 impaired chloroplast division. Thus, the chloroplasts are divided by the PD ring, which is a bundle of PDR1-mediated polyglucan filaments.
巻・号 329(5994)
ページ 949-53
公開日 2010-8-20
DOI 10.1126/science.1190791
PII 329/5994/949
PMID 20724635
MeSH Algal Proteins / genetics Algal Proteins / isolation & purification Algal Proteins / physiology* Chloroplasts / chemistry Chloroplasts / physiology* Chloroplasts / ultrastructure Cytoskeleton / chemistry Cytoskeleton / physiology* Down-Regulation Glucans / isolation & purification Glucans / physiology* Glycosyltransferases / genetics Glycosyltransferases / isolation & purification Glycosyltransferases / physiology* Protein Binding Proteomics Rhodophyta / genetics Rhodophyta / physiology* Rhodophyta / ultrastructure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
IF 41.846
引用数 55
WOS 分野 CELL BIOLOGY
リソース情報
藻類 NIES-3377