Reference - Detail
| RRC ID | 30383 |
|---|---|
| Author | Minoda A, Sakagami R, Yagisawa F, Kuroiwa T, Tanaka K. |
| Title | Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. |
| Journal | Plant Cell Physiol |
| Abstract |
Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination. |
| Volume | 45(6) |
| Pages | 667-71 |
| Published | 2004-6-1 |
| DOI | 10.1093/pcp/pch087 |
| PMID | 15215501 |
| MeSH | Amino Acid Sequence Base Sequence Cell Culture Techniques / methods* Cell Nucleus / genetics* Cells, Cultured Culture Media / pharmacology DNA, Plant / genetics* Molecular Sequence Data Multienzyme Complexes / deficiency Multienzyme Complexes / genetics Mutation / genetics Orotate Phosphoribosyltransferase / deficiency Orotate Phosphoribosyltransferase / genetics Orotic Acid / analogs & derivatives* Orotic Acid / pharmacology Orotidine-5'-Phosphate Decarboxylase / deficiency Orotidine-5'-Phosphate Decarboxylase / genetics Recombination, Genetic / genetics* Rhodophyta / genetics* Rhodophyta / metabolism* Transformation, Genetic / genetics* |
| IF | 4.062 |
| Times Cited | 124 |
| WOS Category | PLANT SCIENCES CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 32 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Algae | NIES-3377 |