RRC ID 30408
Author Moffat KG, Gould JH, Smith HK, O'Kane CJ.
Title Inducible cell ablation in Drosophila by cold-sensitive ricin A chain.
Journal Development
Abstract We have developed a system for temperature-inducible killing of specific cells in the fruitfly Drosophila melanogaster. The system overcomes many of the limitations of existing cell ablation methods and is in principle applicable to any non-homeothermic eukaryote. Temperature-sensitive and cold-sensitive mutations in the ricin toxin A chain (RTA) of castor bean were generated in yeast. One cold-sensitive mutation, RAcs2, produced temperature-dependent ablation of eye cells in Drosophila when expressed under control of the eye-specific sev enhancer. At 29 degrees C, cell death was observed within 7 hours in the developing eye and no obvious toxic effects were observed elsewhere; at 18 degrees C, extremely low toxicity was observed. DNA sequencing of RAcs2 revealed a single amino acid substitution in the RTA active site cleft.
Volume 114(3)
Pages 681-7
Published 1992-3
PMID 1618135
MeSH Animals Cell Death / genetics* Cold Temperature* Drosophila melanogaster / genetics* Eye / cytology Eye / embryology Eye / ultrastructure Genes, Lethal / physiology* Microscopy, Electron, Scanning Ricin / genetics*
IF 5.413
Times Cited 61
Drosophila DGRC#109670