| RRC ID |
30448
|
| Author |
Sano H, Nakamura A, Kobayashi S.
|
| Title |
Identification of a transcriptional regulatory region for germline-specific expression of vasa gene in Drosophila melanogaster.
|
| Journal |
Mech Dev
|
| Abstract |
The vasa gene (vas) is essential for germline development in Drosophila melanogaster. Zygotic vas is expressed in pole cells earlier than any other pole cell-expressing genes thus far identified, and VAS protein is continuously present in germline cells throughout development. Here, we report the identification of a regulatory region that directs germline-specific vas expression. A genomic fragment containing the vas locus was linked to enhanced green fluorescent protein (egfp)-vas fusion gene, and the resulting gene was introduced into fly genome. Developmental vas expression was assessed by monitoring the expression of EGFP-VAS in these transformants. The spatio-temporal expression pattern of EGFP-VAS is essentially identical to that of endogenous VAS throughout germline development. By dissecting the vas promoter, we identified a 40-bp regulatory element, which is necessary and sufficient for germline-specific expression during oogenesis. This region interacts specifically with ovarian protein(s). Furthermore, this region is also required for vas expression in pole cells during embryogenesis. These results suggest that a similar mechanism regulates vas expression both in oogenesis and embryogenesis.
|
| Volume |
112(1-2)
|
| Pages |
129-39
|
| Published |
2002-3-1
|
| DOI |
10.1016/s0925-4773(01)00654-2
|
| PII |
S0925477301006542
|
| PMID |
11850184
|
| MeSH |
5' Untranslated Regions
Animals
Base Sequence
DEAD-box RNA Helicases
Drosophila Proteins
Drosophila melanogaster
Female
Gene Deletion
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
Immunohistochemistry
Luminescent Proteins / metabolism
Microscopy, Fluorescence
Models, Genetic
Molecular Sequence Data
Ovary / metabolism
RNA Helicases / genetics*
Recombinant Fusion Proteins / metabolism
Transcription, Genetic*
beta-Galactosidase / metabolism
|
| IF |
2.126
|
| Times Cited |
64
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Drosophila |
DGRC#109171 |
