Reference - Detail
| RRC ID | 30448 |
|---|---|
| Author | Sano H, Nakamura A, Kobayashi S. |
| Title | Identification of a transcriptional regulatory region for germline-specific expression of vasa gene in Drosophila melanogaster. |
| Journal | Mech Dev |
| Abstract |
The vasa gene (vas) is essential for germline development in Drosophila melanogaster. Zygotic vas is expressed in pole cells earlier than any other pole cell-expressing genes thus far identified, and VAS protein is continuously present in germline cells throughout development. Here, we report the identification of a regulatory region that directs germline-specific vas expression. A genomic fragment containing the vas locus was linked to enhanced green fluorescent protein (egfp)-vas fusion gene, and the resulting gene was introduced into fly genome. Developmental vas expression was assessed by monitoring the expression of EGFP-VAS in these transformants. The spatio-temporal expression pattern of EGFP-VAS is essentially identical to that of endogenous VAS throughout germline development. By dissecting the vas promoter, we identified a 40-bp regulatory element, which is necessary and sufficient for germline-specific expression during oogenesis. This region interacts specifically with ovarian protein(s). Furthermore, this region is also required for vas expression in pole cells during embryogenesis. These results suggest that a similar mechanism regulates vas expression both in oogenesis and embryogenesis. |
| Volume | 112(1-2) |
| Pages | 129-39 |
| Published | 2002-3-1 |
| DOI | 10.1016/s0925-4773(01)00654-2 |
| PII | S0925477301006542 |
| PMID | 11850184 |
| MeSH | 5' Untranslated Regions Animals Base Sequence DEAD-box RNA Helicases Drosophila Proteins Drosophila melanogaster Female Gene Deletion Gene Expression Regulation, Developmental Green Fluorescent Proteins Immunohistochemistry Luminescent Proteins / metabolism Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Ovary / metabolism RNA Helicases / genetics* Recombinant Fusion Proteins / metabolism Transcription, Genetic* beta-Galactosidase / metabolism |
| IF | 2.126 |
| Times Cited | 64 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#109171 |