Reference - Detail
| RRC ID | 30460 |
|---|---|
| Author | Akimoto A, Wada H, Hayashi S. |
| Title | Enhancer trapping with a red fluorescent protein reporter in Drosophila. |
| Journal | Dev Dyn |
| Abstract |
Enhancer trapping allows the detection of genomic transcriptional enhancers and provides a rich source of cell- and region-specific markers in Drosophila. We report here the development of a P-element-based enhancer-trap vector with a nuclear red fluorescent protein (DsRed) reporter for enhancer detection. We demonstrate that this vector can be used for a standard enhancer-trap screen as well as for targeted replacement of previously characterized P-element insertions. We isolated DsRed insertion strains of hedgehog, patched, apterous, teashirt, and dachshund that label specific regions of imaginal discs. The time required for red fluorescence maturation in the eye imaginal disc was estimated to be 22.5 hr. The DsRed markers can be combined with green fluorescent protein markers for double labeling of living Drosophila tissues. |
| Volume | 233(3) |
| Pages | 993-7 |
| Published | 2005-7-1 |
| DOI | 10.1002/dvdy.20439 |
| PMID | 15906373 |
| MeSH | Animals DNA Transposable Elements / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Enhancer Elements, Genetic / genetics* Eye / metabolism Gene Expression Regulation, Developmental / genetics Genes, Reporter / genetics* Genetic Vectors / genetics Kinetics Luminescent Proteins / genetics Luminescent Proteins / metabolism* Nuclear Proteins / metabolism |
| IF | 3.275 |
| Times Cited | 11 |
| WOS Category | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| Resource | |
| Drosophila | DGRC#109135 |