RRC ID 30460
Author Akimoto A, Wada H, Hayashi S.
Title Enhancer trapping with a red fluorescent protein reporter in Drosophila.
Journal Dev Dyn
Abstract Enhancer trapping allows the detection of genomic transcriptional enhancers and provides a rich source of cell- and region-specific markers in Drosophila. We report here the development of a P-element-based enhancer-trap vector with a nuclear red fluorescent protein (DsRed) reporter for enhancer detection. We demonstrate that this vector can be used for a standard enhancer-trap screen as well as for targeted replacement of previously characterized P-element insertions. We isolated DsRed insertion strains of hedgehog, patched, apterous, teashirt, and dachshund that label specific regions of imaginal discs. The time required for red fluorescence maturation in the eye imaginal disc was estimated to be 22.5 hr. The DsRed markers can be combined with green fluorescent protein markers for double labeling of living Drosophila tissues.
Volume 233(3)
Pages 993-7
Published 2005-7-1
DOI 10.1002/dvdy.20439
PMID 15906373
MeSH Animals DNA Transposable Elements / genetics Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Enhancer Elements, Genetic / genetics* Eye / metabolism Gene Expression Regulation, Developmental / genetics Genes, Reporter / genetics* Genetic Vectors / genetics Kinetics Luminescent Proteins / genetics Luminescent Proteins / metabolism* Nuclear Proteins / metabolism
IF 3.275
Times Cited 11
Drosophila DGRC#109135