Reference - Detail
| RRC ID | 30481 |
|---|---|
| Author | Chen CC, Wu JK, Lin HW, Pai TP, Fu TF, Wu CL, Tully T, Chiang AS. |
| Title | Visualizing long-term memory formation in two neurons of the Drosophila brain. |
| Journal | Science |
| Abstract |
Long-term memory (LTM) depends on the synthesis of new proteins. Using a temperature-sensitive ribosome-inactivating toxin to acutely inhibit protein synthesis, we screened individual neurons making new proteins after olfactory associative conditioning in Drosophila. Surprisingly, LTM was impaired after inhibiting protein synthesis in two dorsal-anterior-lateral (DAL) neurons but not in the mushroom body (MB), which is considered the adult learning and memory center. Using a photoconvertible fluorescent protein KAEDE to report de novo protein synthesis, we have directly visualized cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB)-dependent transcriptional activation of calcium/calmodulin-dependent protein kinase II and period genes in the DAL neurons after spaced but not massed training. Memory retention was impaired by blocking neural output in DAL during retrieval but not during acquisition or consolidation. These findings suggest an extra-MB memory circuit in Drosophila: LTM consolidation (MB to DAL), storage (DAL), and retrieval (DAL to MB). |
| Volume | 335(6069) |
| Pages | 678-85 |
| Published | 2012-2-10 |
| DOI | 10.1126/science.1212735 |
| PII | 335/6069/678 |
| PMID | 22323813 |
| MeSH | Animals Animals, Genetically Modified Axons / ultrastructure Brain / cytology Brain / physiology Calcium-Calmodulin-Dependent Protein Kinase Type 2 / biosynthesis Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics Conditioning, Psychological Cryptochromes / biosynthesis Cryptochromes / genetics Cyclic AMP Response Element-Binding Protein / genetics Cyclic AMP Response Element-Binding Protein / metabolism Drosophila / cytology Drosophila / genetics Drosophila / physiology* Drosophila Proteins / biosynthesis* Drosophila Proteins / genetics Drosophila Proteins / metabolism Gene Expression Profiling Gene Expression Regulation Genes, Insect Luminescent Proteins / biosynthesis Luminescent Proteins / genetics Memory, Long-Term / physiology* Mushroom Bodies / physiology* Neurons / physiology* Neurons / ultrastructure Odorants Period Circadian Proteins / biosynthesis Period Circadian Proteins / genetics Ricin / pharmacology Sensory Receptor Cells / physiology Serine Endopeptidases / biosynthesis Serine Endopeptidases / genetics Trans-Activators / genetics Trans-Activators / metabolism Transcriptional Activation |
| IF | 41.846 |
| Times Cited | 101 |
| WOS Category | NEUROSCIENCES |
| Resource | |
| Drosophila | DGRC#109667 DGRC#109670 |