RRC ID 30492
Author Kimura T, Nagao Y, Hashimoto H, Yamamoto-Shiraishi Y, Yamamoto S, Yabe T, Takada S, Kinoshita M, Kuroiwa A, Naruse K.
Title Leucophores are similar to xanthophores in their specification and differentiation processes in medaka.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Animal body color is generated primarily by neural crest-derived pigment cells in the skin. Mammals and birds have only melanocytes on the surface of their bodies; however, fish have a variety of pigment cell types or chromatophores, including melanophores, xanthophores, and iridophores. The medaka has a unique chromatophore type called the leucophore. The genetic basis of chromatophore diversity remains poorly understood. Here, we report that three loci in medaka, namely, leucophore free (lf), lf-2, and white leucophore (wl), which affect leucophore and xanthophore differentiation, encode solute carrier family 2, member 15b (slc2a15b), paired box gene 7a (pax7a), and solute carrier family 2 facilitated glucose transporter, member 11b (slc2a11b), respectively. Because lf-2, a loss-of-function mutant for pax7a, causes defects in the formation of xanthophore and leucophore precursor cells, pax7a is critical for the development of the chromatophores. This genetic evidence implies that leucophores are similar to xanthophores, although it was previously thought that leucophores were related to iridophores, as these chromatophores have purine-dependent light reflection. Our identification of slc2a15b and slc2a11b as genes critical for the differentiation of leucophores and xanthophores in medaka led to a further finding that the existence of these two genes in the genome coincides with the presence of xanthophores in nonmammalian vertebrates: birds have yellow-pigmented irises with xanthophore-like intracellular organelles. Our findings provide clues for revealing diverse evolutionary mechanisms of pigment cell formation in animals.
Volume 111(20)
Pages 7343-8
Published 2014-5-20
DOI 10.1073/pnas.1311254111
PII 1311254111
PMID 24803434
PMC PMC4034200
MeSH Animals Body Patterning Cell Differentiation Chick Embryo Chromatophores / metabolism Chromatophores / physiology* Chromosome Mapping Chromosomes, Artificial, Bacterial / metabolism Gene Expression Regulation, Developmental* Genome Glucose Transport Proteins, Facilitative / metabolism Melanophores / metabolism Molecular Sequence Data Mutation Neural Crest / cytology Neural Crest / pathology Oryzias / embryology* Oryzias / physiology PAX7 Transcription Factor / metabolism Phenotype Phylogeny Pigmentation Vertebrates
IF 9.504
Times Cited 13
WOS Category GENETICS & HEREDITY
Resource
Medaka MT76 MT77 MT104 TG942