論文 - 詳細
| RRC ID | 30563 |
|---|---|
| 著者 | Nagao Y, Suzuki T, Shimizu A, Kimura T, Seki R, Adachi T, Inoue C, Omae Y, Kamei Y, Hara I, Taniguchi Y, Naruse K, Wakamatsu Y, Kelsh RN, Hibi M, Hashimoto H. |
| タイトル | Sox5 functions as a fate switch in medaka pigment cell development. |
| ジャーナル | PLoS Genet |
| Abstract |
Mechanisms generating diverse cell types from multipotent progenitors are crucial for normal development. Neural crest cells (NCCs) are multipotent stem cells that give rise to numerous cell-types, including pigment cells. Medaka has four types of NCC-derived pigment cells (xanthophores, leucophores, melanophores and iridophores), making medaka pigment cell development an excellent model for studying the mechanisms controlling specification of distinct cell types from a multipotent progenitor. Medaka many leucophores-3 (ml-3) mutant embryos exhibit a unique phenotype characterized by excessive formation of leucophores and absence of xanthophores. We show that ml-3 encodes sox5, which is expressed in premigratory NCCs and differentiating xanthophores. Cell transplantation studies reveal a cell-autonomous role of sox5 in the xanthophore lineage. pax7a is expressed in NCCs and required for both xanthophore and leucophore lineages; we demonstrate that Sox5 functions downstream of Pax7a. We propose a model in which multipotent NCCs first give rise to pax7a-positive partially fate-restricted intermediate progenitors for xanthophores and leucophores; some of these progenitors then express sox5, and as a result of Sox5 action develop into xanthophores. Our results provide the first demonstration that Sox5 can function as a molecular switch driving specification of a specific cell-fate (xanthophore) from a partially-restricted, but still multipotent, progenitor (the shared xanthophore-leucophore progenitor). |
| 巻・号 | 10(4) |
| ページ | e1004246 |
| 公開日 | 2014-4-1 |
| DOI | 10.1371/journal.pgen.1004246 |
| PII | PGENETICS-D-13-02764 |
| PMID | 24699463 |
| PMC | PMC3974636 |
| MeSH | Animals Cell Differentiation / genetics Cell Differentiation / physiology Fish Proteins / genetics Gene Expression Regulation, Developmental / genetics Melanophores / physiology Neural Crest / growth & development* Neural Crest / physiology Oryzias / growth & development* Oryzias / physiology PAX7 Transcription Factor / genetics Phenotype Pigmentation / genetics* Pigmentation / physiology SOXD Transcription Factors / genetics* Stem Cells / physiology |
| IF | 5.175 |
| 引用数 | 24 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | sox5^N541S (MT1048) sox5^F543I (MT1049) ml-3 (MT80) lf (MT76) lf-2 (MT77) Tg(slc2a15b-ex1-GFP) (TG942) |