RRC ID 30588
著者 Yoshida K, Treen N, Hozumi A, Sakuma T, Yamamoto T, Sasakura Y.
タイトル Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases.
ジャーナル Genesis
Abstract Targeted mutagenesis of genes-of-interest, or gene-knockout, is a powerful method to address the functions of genes. Engineered nucleases have enabled this approach in various organisms because of their ease of use. The ascidian Ciona intestinalis is an excellent organism to analyze gene functions by means of genetic technologies. In our previous study, we reported mutagenesis of Ciona somatic cells with TALE nucleases (TALENs) by electroporating expression constructs. In this study, we report germ cell mutagenesis of Ciona by microinjecting mRNAs encoding TALENs. TALEN mRNAs introduced mutations to target genes in both somatic and germ cells. TALEN-mediated mutations in the germ cell genome were inherited by the next generation. We conclude that knockout lines of Ciona that have disrupted target genes can be established through TALEN-mediated germ cell mutagenesis.
巻・号 52(5)
ページ 431-9
公開日 2014-5
DOI 10.1002/dvg.22770
PMID 24619765
MeSH Animals Ciona intestinalis / enzymology* Ciona intestinalis / genetics* Deoxyribonucleases / genetics* Deoxyribonucleases / metabolism Electroporation / methods* Gene Knockout Techniques / methods* Genome Germ Cells / metabolism* Mutagenesis
IF 2.143
引用数 13
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int