論文 - 詳細
| RRC ID | 30635 |
|---|---|
| 著者 | Lee HC, Gu W, Shirayama M, Youngman E, Conte D Jr, Mello CC. |
| タイトル | C. elegans piRNAs mediate the genome-wide surveillance of germline transcripts. |
| ジャーナル | Cell |
| Abstract |
Piwi Argonautes and Piwi-interacting RNAs (piRNAs) mediate genome defense by targeting transposons. However, many piRNA species lack obvious sequence complementarity to transposons or other loci; only one C. elegans transposon is a known piRNA target. Here, we show that, in mutants lacking the Piwi Argonaute PRG-1 (and consequently its associated piRNAs/21U-RNAs), many silent loci in the germline exhibit increased levels of mRNA expression with a concomitant depletion of RNA-dependent RNA polymerase (RdRP)-derived secondary small RNAs termed 22G-RNAs. Sequences depleted of 22G-RNAs are proximal to potential target sites that base pair imperfectly but extensively to 21U-RNAs. We show that PRG-1 is required to initiate, but not to maintain, silencing of transgenes engineered to contain complementarity to endogenous 21U-RNAs. Our findings support a model in which C. elegans piRNAs utilize their enormous repertoire of targeting capacity to scan the germline transcriptome for foreign sequences, while endogenous germline-expressed genes are actively protected from piRNA-induced silencing. |
| 巻・号 | 150(1) |
| ページ | 78-87 |
| 公開日 | 2012-7-6 |
| DOI | 10.1016/j.cell.2012.06.016 |
| PII | S0092-8674(12)00765-9 |
| PMID | 22738724 |
| PMC | PMC3410639 |
| MeSH | Animals Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Gene Silencing Genome, Helminth* Germ Cells RNA, Helminth / metabolism* RNA, Small Interfering / metabolism* |
| IF | 38.637 |
| 引用数 | 196 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 |