論文 - 詳細
| RRC ID | 30648 |
|---|---|
| 著者 | Juang BT, Gu C, Starnes L, Palladino F, Goga A, Kennedy S, L'Etoile ND. |
| タイトル | Endogenous nuclear RNAi mediates behavioral adaptation to odor. |
| ジャーナル | Cell |
| Abstract |
Most eukaryotic cells express small regulatory RNAs. The purpose of one class, the somatic endogenous siRNAs (endo-siRNAs), remains unclear. Here, we show that the endo-siRNA pathway promotes odor adaptation in C. elegans AWC olfactory neurons. In adaptation, the nuclear Argonaute NRDE-3, which acts in AWC, is loaded with siRNAs targeting odr-1, a gene whose downregulation is required for adaptation. Concomitant with increased odr-1 siRNA in AWC, we observe increased binding of the HP1 homolog HPL-2 at the odr-1 locus in AWC and reduced odr-1 mRNA in adapted animals. Phosphorylation of HPL-2, an in vitro substrate of the EGL-4 kinase that promotes adaption, is necessary and sufficient for behavioral adaptation. Thus, environmental stimulation amplifies an endo-siRNA negative feedback loop to dynamically repress cognate gene expression and shape behavior. This class of siRNA may act broadly as a rheostat allowing prolonged stimulation to dampen gene expression and promote cellular memory formation. PAPERFLICK: |
| 巻・号 | 154(5) |
| ページ | 1010-1022 |
| 公開日 | 2013-8-29 |
| DOI | 10.1016/j.cell.2013.08.006 |
| PII | S0092-8674(13)00963-X |
| PMID | 23993094 |
| PMC | PMC4274153 |
| MeSH | Adaptation, Physiological Animals Butanones / chemistry Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Chromosomal Proteins, Non-Histone / metabolism Cyclic GMP-Dependent Protein Kinases / metabolism Down-Regulation* Guanylate Cyclase / genetics* Odorants Phosphorylation RNA Interference* RNA, Helminth / metabolism RNA, Small Interfering / metabolism RNA-Binding Proteins / metabolism Sensory Receptor Cells / metabolism* |
| IF | 38.637 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1329 |