論文 - 詳細
| RRC ID | 30663 |
|---|---|
| 著者 | Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, Arita M. |
| タイトル | Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice. |
| ジャーナル | FASEB J |
| Abstract |
Resolution of inflammation is critical to restoration of tissue function after an inflammatory response. We previously demonstrated that 12/15-lipoxygenase (12/15-LOX)-expressing eosinophils contribute to this process in murine zymosan-induced peritonitis. In this study, eosinophils promoted resolution by regulating expression of macrophage CXCL13. Microarray analysis revealed that eosinophils significantly increased (∼3-fold) the expression of macrophage CXCL13 by a 12/15-LOX-dependent mechanism. CXCL13 depletion caused a resolution defect, with the reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph nodes. Inflamed lymph node hypertrophy, a critical feature of the resolution process, was reduced by ∼60% in eosinophil-deficient mice, and adoptive transfer of eosinophils or administration of CXCL13 corrected this defect. Administration of the 12/15-LOX-derived mediator lipoxin A4 (LXA4) increased the expression of CXCL13 and restored the defect of lymph node hypertrophy in eosinophil-deficient mice. These results demonstrate that eosinophils control the resolution of inflammation and draining lymph node hypertrophy through proresolving lipid mediators and the CXCL13 pathway in mice. |
| 巻・号 | 28(9) |
| ページ | 4036-43 |
| 公開日 | 2014-9-1 |
| DOI | 10.1096/fj.14-251132 |
| PII | fj.14-251132 |
| PMID | 24891522 |
| PMC | PMC5395732 |
| MeSH | Animals Arachidonate 12-Lipoxygenase / metabolism* Arachidonate 15-Lipoxygenase / metabolism* Cells, Cultured Chemokine CXCL13 / metabolism* Eosinophils / cytology* Eosinophils / metabolism Flow Cytometry Hypertrophy Inflammation / metabolism Inflammation / pathology* Lipoxins / metabolism Lymph Nodes / metabolism Lymph Nodes / pathology* Macrophages, Peritoneal / metabolism Macrophages, Peritoneal / pathology* Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Microarray Analysis Peritonitis / metabolism Peritonitis / pathology* |
| IF | 4.966 |
| 引用数 | 17 |
| WOS 分野 | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 実験動物マウス | RBRC01908 |