| RRC ID |
30663
|
| 著者 |
Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, Arita M.
|
| タイトル |
Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice.
|
| ジャーナル |
FASEB J
|
| Abstract |
Resolution of inflammation is critical to restoration of tissue function after an inflammatory response. We previously demonstrated that 12/15-lipoxygenase (12/15-LOX)-expressing eosinophils contribute to this process in murine zymosan-induced peritonitis. In this study, eosinophils promoted resolution by regulating expression of macrophage CXCL13. Microarray analysis revealed that eosinophils significantly increased (∼3-fold) the expression of macrophage CXCL13 by a 12/15-LOX-dependent mechanism. CXCL13 depletion caused a resolution defect, with the reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph nodes. Inflamed lymph node hypertrophy, a critical feature of the resolution process, was reduced by ∼60% in eosinophil-deficient mice, and adoptive transfer of eosinophils or administration of CXCL13 corrected this defect. Administration of the 12/15-LOX-derived mediator lipoxin A4 (LXA4) increased the expression of CXCL13 and restored the defect of lymph node hypertrophy in eosinophil-deficient mice. These results demonstrate that eosinophils control the resolution of inflammation and draining lymph node hypertrophy through proresolving lipid mediators and the CXCL13 pathway in mice.
|
| 巻・号 |
28(9)
|
| ページ |
4036-43
|
| 公開日 |
2014-9-1
|
| DOI |
10.1096/fj.14-251132
|
| PII |
fj.14-251132
|
| PMID |
24891522
|
| PMC |
PMC5395732
|
| MeSH |
Animals
Arachidonate 12-Lipoxygenase / metabolism*
Arachidonate 15-Lipoxygenase / metabolism*
Cells, Cultured
Chemokine CXCL13 / metabolism*
Eosinophils / cytology*
Eosinophils / metabolism
Flow Cytometry
Hypertrophy
Inflammation / metabolism
Inflammation / pathology*
Lipoxins / metabolism
Lymph Nodes / metabolism
Lymph Nodes / pathology*
Macrophages, Peritoneal / metabolism
Macrophages, Peritoneal / pathology*
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microarray Analysis
Peritonitis / metabolism
Peritonitis / pathology*
|
| IF |
4.966
|
| 引用数 |
17
|
|
WOS 分野
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC01908 |
