| RRC ID |
30672
|
| 著者 |
Manfruelli P, Reichhart JM, Steward R, Hoffmann JA, Lemaitre B.
|
| タイトル |
A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF.
|
| ジャーナル |
EMBO J
|
| Abstract |
Expression of the gene encoding the antifungal peptide Drosomycin in Drosophila adults is controlled by the Toll signaling pathway. The Rel proteins Dorsal and DIF (Dorsal-related immunity factor) are possible candidates for the transactivating protein in the Toll pathway that directly regulates the drosomycin gene. We have examined the requirement of Dorsal and DIF for drosomycin expression in larval fat body cells, the predominant immune-responsive tissue, using the yeast site-specific flp/FRT recombination system to generate cell clones homozygous for a deficiency uncovering both the dorsal and the dif genes. Here we show that in the absence of both genes, the immune-inducibility of drosomycin is lost but can be rescued by overexpression of either dorsal or dif under the control of a heat-shock promoter. This result suggests a functional redundancy between both Rel proteins in the control of drosomycin gene expression in the larvae of Drosophila. Interestingly, the gene encoding the antibacterial peptide Diptericin remains fully inducible in the absence of the dorsal and dif genes. Finally, we have used fat body cell clones homozygous for various mutations to show that a linear activation cascade Spaetzle--> Toll-->Cactus-->Dorsal/DIF leads to the induction of the drosomycin gene in larval fat body cells.
|
| 巻・号 |
18(12)
|
| ページ |
3380-91
|
| 公開日 |
1999-6-15
|
| DOI |
10.1093/emboj/18.12.3380
|
| PMID |
10369678
|
| PMC |
PMC1171418
|
| MeSH |
Animals
Anti-Infective Agents*
Clone Cells / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / physiology*
Drosophila Proteins*
Drosophila melanogaster / cytology
Drosophila melanogaster / genetics*
Drosophila melanogaster / immunology
Fat Body / metabolism*
Female
Gene Expression Regulation*
Genes, Insect
Genes, Reporter
Insect Proteins / genetics*
Insect Proteins / physiology
Larva / cytology
Larva / genetics
Male
Membrane Glycoproteins / genetics
Membrane Glycoproteins / physiology
Mosaicism
Mutation
Nuclear Proteins / genetics
Nuclear Proteins / physiology*
Phosphoproteins / genetics
Phosphoproteins / physiology*
Receptors, Cell Surface*
Signal Transduction / genetics
Signal Transduction / physiology
Toll-Like Receptors
Transcription Factors*
|
| IF |
9.889
|
| 引用数 |
155
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
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