| RRC ID |
30694
|
| Author |
Oppikofer M, Kueng S, Keusch JJ, Hassler M, Ladurner AG, Gut H, Gasser SM.
|
| Title |
Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.
|
| Journal |
EMBO J
|
| Abstract |
Gene silencing in budding yeast relies on the binding of the Silent Information Regulator (Sir) complex to chromatin, which is mediated by extensive interactions between the Sir proteins and nucleosomes. Sir3, a divergent member of the AAA+ ATPase-like family, contacts both the histone H4 tail and the nucleosome core. Here, we present the structure and function of the conserved C-terminal domain of Sir3, comprising 138 amino acids. This module adopts a variant winged helix-turn-helix (wH) architecture that exists as a stable homodimer in solution. Mutagenesis shows that the self-association mediated by this domain is essential for holo-Sir3 dimerization. Its loss impairs Sir3 loading onto nucleosomes in vitro and eliminates silencing at telomeres and HM loci in vivo. Replacing the Sir3 wH domain with an unrelated bacterial dimerization motif restores both HM and telomeric repression in sir3Δ cells. In contrast, related wH domains of archaeal and human members of the Orc1/Sir3 family are monomeric and have DNA binding activity. We speculate that a dimerization function for the wH evolved with Sir3's ability to facilitate heterochromatin formation.
|
| Volume |
32(3)
|
| Pages |
437-49
|
| Published |
2013-2-6
|
| DOI |
10.1038/emboj.2012.343
|
| PII |
emboj2012343
|
| PMID |
23299941
|
| PMC |
PMC3567499
|
| MeSH |
Amino Acid Sequence
Chromatin / metabolism
Chromatin Immunoprecipitation
Crystallization
DNA Primers / genetics
Dimerization
Evolution, Molecular
Gene Silencing / physiology*
Genetic Complementation Test
Heterochromatin / genetics
Heterochromatin / physiology*
Immunoprecipitation
Models, Molecular*
Molecular Sequence Data
Mutagenesis
Nucleosomes / metabolism
Polymerase Chain Reaction
Protein Conformation*
Saccharomyces cerevisiae
Sequence Alignment
Silent Information Regulator Proteins, Saccharomyces cerevisiae / chemistry
Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics
Silent Information Regulator Proteins, Saccharomyces cerevisiae / metabolism*
|
| IF |
9.889
|
| Times Cited |
18
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| DNA material |
IRAK013J04 (HGX005420). |
