RRC ID |
30725
|
著者 |
Matsumoto S, Fujii S, Sato A, Ibuka S, Kagawa Y, Ishii M, Kikuchi A.
|
タイトル |
A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
|
ジャーナル |
EMBO J
|
Abstract |
Growth factor-dependent epithelial morphological changes and proliferation are essential for the formation of tubular structures, but the underlying molecular mechanisms are poorly understood. Co-stimulation with Wnt3a and epidermal growth factor (Wnt3a/EGF) induced development of tubes consisting of intestinal epithelial cells by inducing expression of Arl4c, an Arf-like small GTP-binding protein, in three-dimensional culture, while stimulation with Wnt3a or EGF alone did not. Arl4c expression resulted in rearrangement of the cytoskeleton through activation of Rac and inactivation of Rho properly, which promoted cell growth by inducing nuclear translocation of Yes-associated protein and transcriptional co-activator with PDZ-binding motif (YAP/TAZ) in leading cells. Arl4c was expressed in ureteric bud tips and pretubular structures in the embryonic kidney. In an organoid culture assay, Wnt and fibroblast growth factor signaling simultaneously induced elongation and budding of kidney ureteric buds through Arl4c expression. YAP/TAZ was observed in the nucleus of extending ureteric bud tips. Thus, Arl4c expression induced by a combination of growth factor signaling mechanisms is involved in tube formation.
|
巻・号 |
33(7)
|
ページ |
702-18
|
公開日 |
2014-4-1
|
DOI |
10.1002/embj.201386942
|
PII |
embj.201386942
|
PMID |
24562386
|
PMC |
PMC4000088
|
MeSH |
ADP-Ribosylation Factors / metabolism*
Acyltransferases
Animals
Cell Adhesion
Cell Differentiation
Cells, Cultured
Cytoskeleton / metabolism
Dogs
Epidermal Growth Factor / metabolism*
Epithelial Cells / cytology
Epithelial Cells / physiology
Female
Fetus
Fibroblast Growth Factors / metabolism
HeLa Cells
Humans
Intestinal Mucosa / metabolism
Intestines / cytology
Kidney / embryology
Madin Darby Canine Kidney Cells
Mice
Mice, Inbred C57BL
Rats
Signal Transduction*
Transcription Factors / metabolism*
Wnt3 Protein / metabolism*
|
IF |
9.889
|
引用数 |
41
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
遺伝子材料 |
B6N BAC Mouse (RDB07573) B6Ng01-089N24
CSII-EF-MCS (RDB04378)
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
CS-RfA-EVBsd (RDB06090)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394). |