| RRC ID |
30743
|
| 著者 |
Ueoka-Nakanishi H, Sazuka T, Nakanishi Y, Maeshima M, Mori H, Hisabori T.
|
| タイトル |
Thioredoxin h regulates calcium dependent protein kinases in plasma membranes.
|
| ジャーナル |
FEBS J
|
| Abstract |
Thioredoxin (Trx) is a key player in redox homeostasis in various cells, modulating the functions of target proteins by catalyzing a thiol-disulfide exchange reaction. Target proteins of cytosolic Trx-h of higher plants were studied, particularly in the plasma membrane, because plant plasma membranes include various functionally important protein molecules such as transporters and signal receptors. Plasma membrane proteins from Arabidopsis thaliana cell cultures were screened using a resin Trx-h1 mutant-immobilized, and a total of 48 candidate proteins obtained. These included two calcium-sensing proteins: a phosphoinositide-specific phospholipase 2 (AtPLC2) and a calcium-dependent protein kinase 21 (AtCPK21). A redox-dependent change in AtCPK21 kinase activity was demonstrated in vitro. Oxidation of AtCPK21 resulted in a decrease in kinase activity to 19% of that of untreated AtCPK21, but Trx-h1 effectively restored the activity to 90%. An intramolecular disulfide bond (Cys97-Cys108) that is responsible for this redox modulation was then identified. In addition, endogenous AtCPK21 was shown to be oxidized in vivo when the culture cells were treated with H2 O2 . These results suggest that redox regulation of AtCPK21 by Trx-h in response to external stimuli is important for appropriate cellular responses. The relationship between the redox regulation system and Ca(2+) signaling pathways is discussed.
|
| 巻・号 |
280(14)
|
| ページ |
3220-31
|
| 公開日 |
2013-7-1
|
| DOI |
10.1111/febs.12301
|
| PMID |
23615222
|
| MeSH |
Amino Acid Sequence
Arabidopsis / enzymology*
Arabidopsis Proteins / chemistry*
Arabidopsis Proteins / metabolism
Arabidopsis Proteins / physiology*
Calcium Signaling
Cell Membrane / enzymology*
Cells, Cultured
Cystine / chemistry
Dithiothreitol / chemistry
Hydrogen Peroxide / pharmacology
Molecular Sequence Data
Oxidants / pharmacology
Oxidation-Reduction
Protein Kinases / chemistry*
Protein Kinases / metabolism
Reducing Agents / chemistry
Thioredoxin h / chemistry
Thioredoxin h / physiology*
Two-Hybrid System Techniques
|
| IF |
4.392
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
rpc00008 |
