RRC ID 30766
著者 Tominaga M, Kimura A, Yokota E, Haraguchi T, Shimmen T, Yamamoto K, Nakano A, Ito K.
タイトル Cytoplasmic streaming velocity as a plant size determinant.
ジャーナル Dev Cell
Abstract Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
巻・号 27(3)
ページ 345-52
公開日 2013-11-11
DOI 10.1016/j.devcel.2013.10.005
PII S1534-5807(13)00602-3
PMID 24229646
MeSH Actins / genetics Actins / metabolism* Arabidopsis / growth & development* Arabidopsis / metabolism* Cell Size* Cytoplasmic Streaming / physiology* Immunoblotting Myosins / genetics Myosins / metabolism* Plants, Genetically Modified Protoplasts / metabolism RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction
IF 10.092
引用数 57
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda01455