RRC ID 30766
Author Tominaga M, Kimura A, Yokota E, Haraguchi T, Shimmen T, Yamamoto K, Nakano A, Ito K.
Title Cytoplasmic streaming velocity as a plant size determinant.
Journal Dev Cell
Abstract Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
Volume 27(3)
Pages 345-52
Published 2013-11-11
DOI 10.1016/j.devcel.2013.10.005
PII S1534-5807(13)00602-3
PMID 24229646
MeSH Actins / genetics Actins / metabolism* Arabidopsis / growth & development* Arabidopsis / metabolism* Cell Size* Cytoplasmic Streaming / physiology* Immunoblotting Myosins / genetics Myosins / metabolism* Plants, Genetically Modified Protoplasts / metabolism RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction
IF 10.092
Times Cited 57
Arabidopsis / Cultured plant cells, genes pda01455