論文 - 詳細
| RRC ID | 30767 |
|---|---|
| 著者 | Iwakawa HO, Tomari Y. |
| タイトル | Molecular insights into microRNA-mediated translational repression in plants. |
| ジャーナル | Mol Cell |
| Abstract |
microRNAs (miRNAs) bind Argonaute proteins in order to form RNA-induced silencing complexes (RISCs) that can silence the expression of complementary mRNAs. Plant miRNAs can mediate the cleavage of their target mRNAs as well as the repression of their translation. Here, by using an in vitro system prepared from plant culture cells, we biochemically dissect the mechanisms by which Arabidopsis thaliana ARGONAUTE1 RISC (AtAGO1-RISC) silences its mRNA targets. We find that AtAGO1-RISC has the ability to repress translation initiation without promoting deadenylation or mRNA decay. Strikingly, AtAGO1-RISC bound in the 5' untranslated region or the open reading frame can sterically block the recruitment or movement of ribosomes. These silencing effects require more extensive base pairing to the target site in comparison to typical animal miRNAs. Our data provide mechanistic insights into miRNA-mediated translational repression in plants. |
| 巻・号 | 52(4) |
| ページ | 591-601 |
| 公開日 | 2013-11-21 |
| DOI | 10.1016/j.molcel.2013.10.033 |
| PII | S1097-2765(13)00799-5 |
| PMID | 24267452 |
| MeSH | 3' Untranslated Regions 5' Untranslated Regions Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Base Sequence Cells, Cultured Gene Expression Regulation, Plant* MicroRNAs / genetics* Open Reading Frames Polyadenylation Protein Binding Protein Biosynthesis* RNA Interference* RNA Stability RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Plant / genetics RNA, Plant / metabolism RNA-Induced Silencing Complex / metabolism Tobacco |
| IF | 15.584 |
| 引用数 | 121 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda04693 pda10504 |