RRC ID 30767
Author Iwakawa HO, Tomari Y.
Title Molecular insights into microRNA-mediated translational repression in plants.
Journal Mol. Cell
Abstract microRNAs (miRNAs) bind Argonaute proteins in order to form RNA-induced silencing complexes (RISCs) that can silence the expression of complementary mRNAs. Plant miRNAs can mediate the cleavage of their target mRNAs as well as the repression of their translation. Here, by using an in vitro system prepared from plant culture cells, we biochemically dissect the mechanisms by which Arabidopsis thaliana ARGONAUTE1 RISC (AtAGO1-RISC) silences its mRNA targets. We find that AtAGO1-RISC has the ability to repress translation initiation without promoting deadenylation or mRNA decay. Strikingly, AtAGO1-RISC bound in the 5' untranslated region or the open reading frame can sterically block the recruitment or movement of ribosomes. These silencing effects require more extensive base pairing to the target site in comparison to typical animal miRNAs. Our data provide mechanistic insights into miRNA-mediated translational repression in plants.
Volume 52(4)
Pages 591-601
Published 2013-11-21
DOI 10.1016/j.molcel.2013.10.033
PII S1097-2765(13)00799-5
PMID 24267452
MeSH 3' Untranslated Regions 5' Untranslated Regions Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Base Sequence Cells, Cultured Gene Expression Regulation, Plant* MicroRNAs / genetics* Open Reading Frames Polyadenylation Protein Binding Protein Biosynthesis* RNA Interference* RNA Stability RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Plant / genetics RNA, Plant / metabolism RNA-Induced Silencing Complex / metabolism Tobacco
IF 14.248
Times Cited 69
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda04693 pda10504