Reference - Detail
| RRC ID | 30767 |
|---|---|
| Author | Iwakawa HO, Tomari Y. |
| Title | Molecular insights into microRNA-mediated translational repression in plants. |
| Journal | Mol Cell |
| Abstract |
microRNAs (miRNAs) bind Argonaute proteins in order to form RNA-induced silencing complexes (RISCs) that can silence the expression of complementary mRNAs. Plant miRNAs can mediate the cleavage of their target mRNAs as well as the repression of their translation. Here, by using an in vitro system prepared from plant culture cells, we biochemically dissect the mechanisms by which Arabidopsis thaliana ARGONAUTE1 RISC (AtAGO1-RISC) silences its mRNA targets. We find that AtAGO1-RISC has the ability to repress translation initiation without promoting deadenylation or mRNA decay. Strikingly, AtAGO1-RISC bound in the 5' untranslated region or the open reading frame can sterically block the recruitment or movement of ribosomes. These silencing effects require more extensive base pairing to the target site in comparison to typical animal miRNAs. Our data provide mechanistic insights into miRNA-mediated translational repression in plants. |
| Volume | 52(4) |
| Pages | 591-601 |
| Published | 2013-11-21 |
| DOI | 10.1016/j.molcel.2013.10.033 |
| PII | S1097-2765(13)00799-5 |
| PMID | 24267452 |
| MeSH | 3' Untranslated Regions 5' Untranslated Regions Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Base Sequence Cells, Cultured Gene Expression Regulation, Plant* MicroRNAs / genetics* Open Reading Frames Polyadenylation Protein Binding Protein Biosynthesis* RNA Interference* RNA Stability RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Plant / genetics RNA, Plant / metabolism RNA-Induced Silencing Complex / metabolism Tobacco |
| IF | 15.584 |
| Times Cited | 121 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda04693 pda10504 |