| RRC ID |
30767
|
| 著者 |
Iwakawa HO, Tomari Y.
|
| タイトル |
Molecular insights into microRNA-mediated translational repression in plants.
|
| ジャーナル |
Mol Cell
|
| Abstract |
microRNAs (miRNAs) bind Argonaute proteins in order to form RNA-induced silencing complexes (RISCs) that can silence the expression of complementary mRNAs. Plant miRNAs can mediate the cleavage of their target mRNAs as well as the repression of their translation. Here, by using an in vitro system prepared from plant culture cells, we biochemically dissect the mechanisms by which Arabidopsis thaliana ARGONAUTE1 RISC (AtAGO1-RISC) silences its mRNA targets. We find that AtAGO1-RISC has the ability to repress translation initiation without promoting deadenylation or mRNA decay. Strikingly, AtAGO1-RISC bound in the 5' untranslated region or the open reading frame can sterically block the recruitment or movement of ribosomes. These silencing effects require more extensive base pairing to the target site in comparison to typical animal miRNAs. Our data provide mechanistic insights into miRNA-mediated translational repression in plants.
|
| 巻・号 |
52(4)
|
| ページ |
591-601
|
| 公開日 |
2013-11-21
|
| DOI |
10.1016/j.molcel.2013.10.033
|
| PII |
S1097-2765(13)00799-5
|
| PMID |
24267452
|
| MeSH |
3' Untranslated Regions
5' Untranslated Regions
Arabidopsis / genetics*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Base Sequence
Cells, Cultured
Gene Expression Regulation, Plant*
MicroRNAs / genetics*
Open Reading Frames
Polyadenylation
Protein Binding
Protein Biosynthesis*
RNA Interference*
RNA Stability
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Plant / genetics
RNA, Plant / metabolism
RNA-Induced Silencing Complex / metabolism
Tobacco
|
| IF |
15.584
|
| 引用数 |
121
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda04693
pda10504 |
