Reference - Detail
| RRC ID | 30796 |
|---|---|
| Author | Enoki Y, Sato T, Tanaka S, Iwata T, Usui M, Takeda S, Kokabu S, Matsumoto M, Okubo M, Nakashima K, Yamato M, Okano T, Fukuda T, Chida D, Imai Y, Yasuda H, Nishihara T, Akita M, Oda H, Okazaki Y, Suda T, Yoda T. |
| Title | Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo. |
| Journal | FEBS Lett |
| Abstract |
Bone is a highly vascularized organ, thus angiogenesis is a vital process during bone remodeling. However, the role of vascular systems in bone remodeling is not well recognized. Here we show that netrin-4 inhibits osteoclast differentiation in vitro and in vivo. Co-cultures of bone marrow macrophages with vascular endothelial cells markedly inhibited osteoclast differentiation. Adding a neutralizing antibody, or RNA interference against netrin-4, restored in vitro osteoclast differentiation. Administration of netrin-4 prevented bone loss in an osteoporosis mouse model by decreasing the osteoclast number. We propose that vascular endothelial cells interact with bone in suppressing bone through netrin-4. |
| Volume | 588(14) |
| Pages | 2262-9 |
| Published | 2014-6-27 |
| DOI | 10.1016/j.febslet.2014.05.009 |
| PII | S0014-5793(14)00370-6 |
| PMID | 24846137 |
| MeSH | Animals Bone Resorption / metabolism Cell Differentiation* Cells, Cultured Coculture Techniques Endothelial Cells / metabolism* Endothelium, Vascular / pathology Macrophages / metabolism Mice Mice, Inbred C57BL Nerve Growth Factors / physiology* Netrins Osteoclasts / physiology* Osteoporosis / chemically induced Osteoporosis / metabolism* RANK Ligand |
| IF | 3.057 |
| Times Cited | 17 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | UV♀2(RCB1994) |