論文 - 詳細
| RRC ID | 30831 |
|---|---|
| 著者 | Yasuoka Y, Suzuki Y, Takahashi S, Someya H, Sudou N, Haramoto Y, Cho KW, Asashima M, Sugano S, Taira M. |
| タイトル | Occupancy of tissue-specific cis-regulatory modules by Otx2 and TLE/Groucho for embryonic head specification. |
| ジャーナル | Nat Commun |
| Abstract |
Head specification by the head-selector gene, orthodenticle (otx), is highly conserved among bilaterian lineages. However, the molecular mechanisms by which Otx and other transcription factors (TFs) interact with the genome to direct head formation are largely unknown. Here we employ ChIP-seq and RNA-seq approaches in Xenopus tropicalis gastrulae and find that occupancy of the corepressor, TLE/Groucho, is a better indicator of tissue-specific cis-regulatory modules (CRMs) than the coactivator p300, during early embryonic stages. On the basis of TLE binding and comprehensive CRM profiling, we define two distinct types of Otx2- and TLE-occupied CRMs. Using these devices, Otx2 and other head organizer TFs (for example, Lim1/Lhx1 (activator) or Goosecoid (repressor)) are able to upregulate or downregulate a large battery of target genes in the head organizer. An underlying principle is that Otx marks target genes for head specification to be regulated positively or negatively by partner TFs through specific types of CRMs. |
| 巻・号 | 5 |
| ページ | 4322 |
| 公開日 | 2014-7-9 |
| DOI | 10.1038/ncomms5322 |
| PII | ncomms5322 |
| PMID | 25005894 |
| PMC | PMC4805429 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism* Binding Sites Female Gastrula / embryology Gastrula / metabolism Gene Expression Regulation, Developmental* Head / embryology* Male Organ Specificity Otx Transcription Factors / genetics Otx Transcription Factors / metabolism* Promoter Regions, Genetic Protein Binding Transcription Factors / genetics Transcription Factors / metabolism Xenopus / embryology Xenopus / genetics Xenopus / metabolism* Xenopus Proteins / genetics Xenopus Proteins / metabolism* |
| IF | 12.121 |
| 引用数 | 30 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ツメガエル・イモリ | X. tropicalis |